Western Blot (WB)
(Western blot analysis of FAPP1 antibody (C-term) in Ramos cell line lysates (35ug/lane). FAPP1 (arrow) was detected using the purified Pab.)
Rabbit anti-Human FAPP1 Polyclonal Antibody | anti-MIS18A antibody
FAPP1, CT (Pleckstrin Homology Domain-containing Family A Member 3, PH Domain-containing Family A Member 3, Phosphoinositol 4-phosphate Adaptor Protein 1, Phosphoinositol 4-phosphate Adapter Protein 1, FAPP-1, PLEKHA3)
Gene Names
MIS18A; B28; FASP1; C21orf45; C21orf46; MIS18alpha; hMis18alpha
Reactivity
Human
Applications
Flow Cytometry, Western Blot, Immunohistochemistry
Purity
PurifiedPurified by ammonium sulfate precipitation.
Synonyms
FAPP1; Polyclonal Antibody; CT (Pleckstrin Homology Domain-containing Family A Member 3; PH Domain-containing Family A Member 3; Phosphoinositol 4-phosphate Adaptor Protein 1; Phosphoinositol 4-phosphate Adapter Protein 1; FAPP-1; PLEKHA3); Anti -FAPP1; anti-MIS18A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human FAPP1.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Concentration
2mg/ml (varies by lot)
Applicable Applications for anti-MIS18A antibody
Flow Cytometry (FC), Western Blot (WB), Immunohistochemistry (IHC0
Application Notes
Suitable for use in Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Dilution:
Western Blot: 1:1000
Immunohistochemistry (paraffin):1:50-100
Flow Cytometry: 1:10-50
Dilution:
Western Blot: 1:1000
Immunohistochemistry (paraffin):1:50-100
Flow Cytometry: 1:10-50
Immunogen
Synthetic peptide selected from the C-terminal region of human FAPP1 (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of FAPP1 antibody (C-term) in Ramos cell line lysates (35ug/lane). FAPP1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of FAPP1 antibody (C-term) in Ramos cell line lysates (35ug/lane). FAPP1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma with FAPP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma with FAPP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of hela cells using FAPP1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of hela cells using FAPP1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-MIS18A antibody
FAPP1 binds specifically to phosphatidylinositol-4-phosphate (PtdIns4P), but not to other phosphoinositides.
Product Categories/Family for anti-MIS18A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
25,863 Da
NCBI Official Full Name
FAPP1-associated protein 1
NCBI Official Synonym Full Names
MIS18 kinetochore protein homolog A (S. pombe)
NCBI Official Symbol
MIS18A
NCBI Official Synonym Symbols
B28; FASP1; C21orf45; C21orf46; MIS18alpha; hMis18alpha
NCBI Protein Information
protein Mis18-alpha; OTTHUMP00000107699; FAPP1-associated protein 1
UniProt Protein Name
Protein Mis18-alpha
Protein Family
UniProt Gene Name
MIS18A
UniProt Synonym Gene Names
C21orf45; C21orf46; FASP1
UniProt Entry Name
MS18A_HUMAN
Uniprot Description
MIS18A: Required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis. Belongs to the mis18 family.
Protein type: Cell cycle regulation
Chromosomal Location of Human Ortholog: 21q22.11
Cellular Component: nucleoplasm; cytoplasm; nucleus; chromosome, pericentric region
Molecular Function: protein binding
Biological Process: mitosis; nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; cell division; chromosome segregation
Research Articles on MIS18A
Similar Products
Product Notes
The MIS18A mis18a (Catalog #AAA626860) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FAPP1, CT (Pleckstrin Homology Domain-containing Family A Member 3, PH Domain-containing Family A Member 3, Phosphoinositol 4-phosphate Adaptor Protein 1, Phosphoinositol 4-phosphate Adapter Protein 1, FAPP-1, PLEKHA3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FAPP1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Western Blot (WB), Immunohistochemistry (IHC0. Suitable for use in Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested. Dilution: Western Blot: 1:1000 Immunohistochemistry (paraffin):1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the MIS18A mis18a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAPP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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