Western Blot (WB)
Mouse anti-Human Mad1 Monoclonal Antibody | anti-Mad1 antibody
Mouse Anti-Mad1 [9B10]
Reactivity
Human
Applications
Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
Mad1; Monoclonal Antibody; Mouse Anti-Mad1 [9B10]; hMAD1 antibody; HsMAD1 antibody; MAD-1 antibody; MAD1L1 antibody; Mitotic arrest deficient 1antibody; Mitotic checkpoint MAD1 protein homolog antibody; Mitotic spindle assembly checkpoint protein MAD1antibody; PIG-9 antibody; PIG9 antibody; Tax binding protein 181 antibody; TP53I9 antibody; Tumor protein p53inducible protein 9 antibody; TXBP181 antibody; anti-Mad1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
9B10
Purity/Purification
Protein A
Form/Format
Purified antibody containing PBS 0.1% sodium azide
Concentration
0.9mg/ml (varies by lot)
Applicable Applications for anti-Mad1 antibody
Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
Optimal antibody dilution should be determined by titration, however as a guideline try; IF: 1/500 - 1/1000. IP: Use at a concentration of 5 ug/0.5 - 1ml of extract.
Target Antigen
Mad1
Immunogen
Recombinant full length Mad1 protein (human)
Research Areas
HLH / Leucine Zipper; Transcription; Tumor Suppressors
Western Blot (WB)
Western Blot (WB)
References
Centromere associated proteinE is essential for the mammalian mitotic checkpoint to prevent aneuploidy due to single chromosome loss. Weaver BA (J Cell Biol,Aug 2003) PUBMED Hewitt L et al. Sustained Mps1 activity is required in mitosis to recruit O-Mad2 to the Mad1-C-Mad2 core complex. J Cell Biol 190:25-34 (2010). ICC/IF ; Human PUBMED
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Product Notes
The Mad1 (Catalog #AAA375007) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Mad1 [9B10] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Mad1 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). Optimal antibody dilution should be determined by titration, however as a guideline try; IF: 1/500 - 1/1000. IP: Use at a concentration of 5 ug/0.5 - 1ml of extract. Researchers should empirically determine the suitability of the Mad1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mad1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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