Rabbit anti-Human MRP5 Polyclonal Antibody | anti-MRP5 antibody

MRP5, ID (Multidrug Resistance-associated Protein 5, ATP-binding Cassette Sub-family C Member 5, Multi-specific Organic Anion Transporter C, MOAT-C, pABC11, SMRP, ABCC5) (FITC)

Cat. Num. AAA6488602

• Gene Names: ABCC5; MRP5; SMRP; ABC33; MOATC; MOAT-C; pABC11; EST277145
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: MRP5; Polyclonal Antibody; ID (Multidrug Resistance-associated Protein 5; ATP-binding Cassette Sub-family C Member 5; Multi-specific Organic Anion Transporter C; MOAT-C; pABC11; SMRP; ABCC5) (FITC); anti-MRP5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ABCC5.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-MRP5 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human ABCC5 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ABCC5 Antibody (Center) in 293 cell line lysates (35ug/lane). ABCC5 (arrow) was detected using the purified Pab.(2ug/ml))

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue with ABCC5 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of 293 cells using ABCC5 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-MRP5 antibody

Antibodies; Multi Drug Resistance (MDR)

References

1. Olsen, J.V., et.al., Cell 127(3), 635-648 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 66529005
• NCBI GeneID: 10057
• NCBI Accession #: NP_005679.2
• NCBI GenBank Nucleotide #: NM_005688.2
• UniProt Accession #: O15440; O14517; Q29ZA9; Q29ZB1; Q86UX3; Q86W30; Q9UN85; Q9UNP5; Q9UQC3; B9EIQ2
• Molecular Weight: 155,732 Da
• NCBI Official Full Name: multidrug resistance-associated protein 5 isoform 1
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family C (CFTR/MRP), member 5
• NCBI Official Symbol: ABCC5
• NCBI Official Synonym Symbols: MRP5; SMRP; ABC33; MOATC; MOAT-C; pABC11; EST277145
• NCBI Protein Information: multidrug resistance-associated protein 5; ATP-binding cassette sub-family C member 5; multi-specific organic anion transporter C; canalicular multispecific organic anion transporter C
• UniProt Protein Name: Multidrug resistance-associated protein 5
• Protein Family: Probable 37S ribosomal protein
• UniProt Gene Name: ABCC5
• UniProt Synonym Gene Names: MRP5; MOAT-C
• UniProt Entry Name: MRP5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions in the cellular export of its substrate, cyclic nucleotides. This export contributes to the degradation of phosphodiesterases and possibly an elimination pathway for cyclic nucleotides. Studies show that this protein provides resistance to thiopurine anticancer drugs, 6-mercatopurine and thioguanine, and the anti-HIV drug 9-(2-phosphonylmethoxyethyl)adenine. This protein may be involved in resistance to thiopurines in acute lymphoblastic leukemia and antiretroviral nucleoside analogs in HIV-infected patients. Alternative splicing of this gene has been detected; however, the complete sequence and translation initiation site is unclear. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCC5: Acts as a multispecific organic anion pump which can transport nucleotide analogs. Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ABC family

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: membrane; integral to plasma membrane; plasma membrane

Molecular Function: ATPase activity, coupled to transmembrane movement of substances; organic anion transmembrane transporter activity; ATP binding

Biological Process: glycosaminoglycan metabolic process; transport; hyaluronan biosynthetic process; carbohydrate metabolic process; pathogenesis; transmembrane transport; hyaluronan metabolic process; organic anion transport