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Western Blot (WB) ((2ug/ml) staining of Mouse (A) and Rat (B) Eye lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)

Goat RNF13/RZF Polyclonal Antibody | anti-RNF13 antibody

Goat anti-RNF13/RZF Antibody

Gene Names
RNF13; RZF
Reactivity
Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
RNF13/RZF; Polyclonal Antibody; Goat anti-RNF13/RZF Antibody; Goat Anti-RNF13 / RZF (isoform 1 and 3) Antibody; RNF13; ring finger protein 13; MGC13689; RZF; RING zinc finger protein; RNF13 antibody; ring finger protein 13 antibody; MGC13689 antibody; RZF antibody; RING zinc finger protein antibody; RNF13 / RZF; anti-RNF13 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
HKFKKGDEYDVC
Sequence Length
381
Applicable Applications for anti-RNF13 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 2000.
Western Blot: Approx 45kDa band observed in Mouse Eye and in Rat Retina lysates (calculated MW of 42.8kDa according to NP_009213.1). Recommended concentration: 2-4ug/ml.
Immunogen
Peptide with sequence HKFKKGDEYDVC, from the internal region of the protein sequence according to NP_009213.1; NP_899237.1; NP_899239.2; NP_899240.2.
Epitope
Internal region
Note
This antibody is expected to recognize all reported isoforms (NP_009213.1, NP_899239.2; NP_899240.2).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((2ug/ml) staining of Mouse (A) and Rat (B) Eye lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)

Western Blot (WB) ((2ug/ml) staining of Mouse (A) and Rat (B) Eye lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
Molecular Weight
29,713 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase RNF13
NCBI Official Synonym Full Names
ring finger protein 13
NCBI Official Symbol
RNF13
NCBI Official Synonym Symbols
RZF
NCBI Protein Information
E3 ubiquitin-protein ligase RNF13
UniProt Protein Name
E3 ubiquitin-protein ligase RNF13
UniProt Gene Name
RNF13
UniProt Synonym Gene Names
RZF
UniProt Entry Name
RNF13_HUMAN

NCBI Description

The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. The specific function of this gene has not yet been determined. Alternatively spliced transcript variants that encode the same protein have been reported. A pseudogene, which is also located on chromosome 3, has been defined for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

RNF13: E3 ubiquitin-protein ligase that may play a role in controlling cell proliferation.

Protein type: Ligase; EC 6.3.2.-; EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q25.1

Cellular Component: endoplasmic reticulum membrane; Golgi membrane; integral to membrane; late endosome membrane; lysosomal membrane; nuclear inner membrane

Molecular Function: ligase activity; protein binding; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: protein autoubiquitination

Research Articles on RNF13

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Product Notes

The RNF13 rnf13 (Catalog #AAA423388) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-RNF13/RZF Antibody reacts with Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's RNF13/RZF can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 2000. Western Blot: Approx 45kDa band observed in Mouse Eye and in Rat Retina lysates (calculated MW of 42.8kDa according to NP_009213.1). Recommended concentration: 2-4ug/ml. Researchers should empirically determine the suitability of the RNF13 rnf13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: HKFKKGDEYD VC. It is sometimes possible for the material contained within the vial of "RNF13/RZF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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