Rabbit FAPbeta Polyclonal Antibody | anti-FAPbeta antibody

Anti-FAPbeta (Fibroblast Activation Protein alpha Subunit, Seprase, Integral Membrane Serine Protease) Spacer Region Antibody

Cat. Num. AAA4157555

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: FAPbeta; Polyclonal Antibody; Anti-FAPbeta (Fibroblast Activation Protein alpha Subunit; Seprase; Integral Membrane Serine Protease) Spacer Region Antibody; anti-FAPbeta antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody recognizes both long isoforms of Fibroblast activation protein, alpha, but does not recognize other DPP family members.
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)

• Applicable Applications for anti-FAPbeta antibody: Western Blot (WB)
• Application Notes: WB: 1:1000 when using colorimetric substrates such as BCIP/NBT and 1:5000 for chemiluminescent substrates. Detects a band of approximately 110-120 kDa due to post translational modifications and reduction. (Predicted molecular weight: 88 kDa). Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.
• Immunogen: Synthetic peptide of Human Fibroblast activation protein, alpha based on the spacer region.
• Cellular Localization: Cell Membrane; Single-Pass Type II Membrane Protein. Found in cell surface lamellipodia, invadopodia and on shed vesicles.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-FAPbeta antibody

Description: The antibody is a rabbit polyclonal antibody made to the Type-II serine protease FAPbeta.. The antibody is made to a synthetic peptide based on the spacer region of human FAPbeta. The antibody reacts with human, mouse and rat FAPbeta, and does not recognize other DPP family members. The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant. FAPbeta (Fibroblast Activation Protein alpha subunit) is the alpha subunit and DPP-IV the beta subunit of a heterodimeric membrane-bound proteinase complex. Also known as 170 kDa Melanoma Membrane Gelatinase, Integral Membrane Serine Proteinase and Seprase, FAPbeta was first identified as a membrane glycoprotein of fibroblasts and tumor cells, in a search for tumor cell markers. The beta subunit of the complex was identified as dipeptidyl peptidase-4 (DPP-IV), also known as CD26. Not all cells and tissues make the heterodimer; some make only FAPbeta homodimers, some only DPP-4 homodimers. It has been reported that CD26/FAPbeta colocalizes with UPAR in melanoma cells, that larger complexes form with integrins and other connective tissue components, and that this impacts cell migration. Also reported is that FAPbeta is produced by over 90% of human epithelial cancers, expressed by the stromal fibroblasts. FAPbeta also is reported to promote tumor growth and angiogenesis in breast cancer model systems. FAPbeta at the edges of invadipodia is thought to aid in cell migration and ECM/connective tissue breakdown. FAPbeta is considered a dual-function proteinase, acting both as a dipeptidyl peptidase and as a collagenase, although there is some debate as to specificity. The clan SC proteinases have a catalytic triad of Ser-Asp-His, and like other Serine proteinases, the active site serine is in a Gly-Xaa-Ser-Xaa-Gly orientation. FAPbeta is a member of a broader family of dipeptidyl peptidases including DPP-4, DPP-2, DPP-8, DPP-9, DPP-10, which have differing substrate specificities and tissue localizations. The surface-bound FAPbeta is shed from cells, and cleavage of the stalk region releases the shed form found in serum. Two human FAPbeta have been reported, 759 and 760 amino acids in length, with predicted mass of 87.7 and 87.8 kDa, and pI of 6.2 and 6.45 respectively. Postranslational modifications and reduction lead to an apparent mass of 110-120 kDa on SDS-PAGE gels. The 759 amino acid sequence has an insertion in the catalytic domain, but retains the catalytic triad. It is not clear what functional difference the two isoforms have. The FAPbeta antibody recognizes both the 759 and 760 amino acid forms. There also is reported a much shorter sequence encoding only the carboxyterminal portion of FAPbeta, only the catalytic domain. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.

Relevance: Fibroblast Activation Protein alpha subunit (FAPa or FAP, alpha) is a integral membrane gelatinase belonging to the serine protease family. FAPa is the alpha subunit and DPP4 (CD26) the beta subunit of a heterodimeric membrane-bound proteinase complex also known as 170 kDa Melanoma Membrane Gelatinase, Integral Membrane Serine Proteinase and Seprase. Some cells make only FAPa homodimers, some only DPP4 homodimers. The monomer is inactive. FAP, alpha is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis.