Testing Data
(C2C12 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with MBS633220 (1:1000 dilution). Proteins were visualized using a|goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein integrin b1 (~130kD).)
Rat anti-Mouse Integrin, beta1 Monoclonal Antibody | anti-ITGB1 antibody
Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla)
Purified by Protein A chromatography.
Purified by Protein A chromatography.
Dilution: Immunoprecipitation: 2ug; lysate of 10e6 cell
Flow Cytometry: 10ug/ml
Immunohistochemistry (Frozen): 20ug/ml. As a result of the wide tissue distribution of VLA (b1), binding to diverse cell types is commonly observed.
Western Blot: 1:1000 on 10ug C2C12 lysate. C2C12 lysates was resolved by electrophoresis, transferred to PDVF membrane and probed with I7661-39J. Proteins were visualized using an IgG (HRP) goat anti-rat and a chemiluminescent detection system.
Inhibition: Does not block VLA integrin mediated cell adhesion to matrix proteins.
Optimal dilution determined by the researcher.
Testing Data
(C2C12 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with MBS633220 (1:1000 dilution). Proteins were visualized using a|goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein integrin b1 (~130kD).)
Testing Data
(C2C12 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with MBS633220 (1:1000 dilution). Proteins were visualized using a|goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein integrin b1 (~130kD).)
NCBI and Uniprot Product Information
NCBI Description
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq]
Uniprot Description
ITGB1: an integral membrane protein that heterodimerizes with an alpha-3 chain, forming a receptor for many extracellular-matrix proteins including fibronectin, laminin, collagen, epiligrin and thrombospondin. . Beta 1 integrins recognize the amino-acid motif RGD in a wide array of ligands. Five alternatively spliced variants with alternate carboxy termini have been described. Two alternatively spliced isoforms have been described. Isoform beta-1a is widely expressed; other isoforms are generally expressed with a more restricted distribution. Isoform beta-1b is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoforms beta-1c and beta-1c-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-c-2, rather than isoform beta-1c, is selectively expressed in primary t-cells. Isoform beta-1c is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1d is expressed specifically in striated muscle (skeletal and cardiac muscle).
Protein type: Motility/polarity/chemotaxis; Cell adhesion; Cell surface; Membrane protein, integral; Receptor, misc.
Chromosomal Location of Human Ortholog: 10p11.2
Cellular Component: focal adhesion; cell surface; acrosome; dendritic spine; lipid raft; ruffle; recycling endosome; membrane; hemidesmosome; lamellipodium; cytoplasm; plasma membrane; melanosome; basement membrane; neuromuscular junction; integrin complex; receptor complex; sarcolemma; cleavage furrow; external side of plasma membrane; filopodium
Molecular Function: protein domain specific binding; protease binding; metal ion binding; laminin binding; actin binding; alpha-actinin binding; peptide binding; protein kinase binding; viral receptor activity; integrin binding; protein binding; protein heterodimerization activity; fibronectin binding; cell adhesion molecule binding; glycoprotein binding
Biological Process: axon guidance; extracellular matrix organization and biogenesis; entry of virus into host cell; maternal process involved in pregnancy; regulation of cell cycle; positive regulation of apoptosis; axon extension; cell-matrix adhesion; mesodermal cell differentiation; cell fate specification; positive regulation of endocytosis; cardiac muscle cell differentiation; negative regulation of cell proliferation; leukocyte adhesion; positive regulation of MAPKKK cascade; transforming growth factor beta receptor signaling pathway; germ cell migration; positive regulation of cell proliferation; tissue homeostasis; visual learning; negative regulation of cell projection organization and biogenesis; response to drug; integrin-mediated signaling pathway; cell migration; regulation of immune response; in utero embryonic development; dendrite morphogenesis; sarcomere organization; protein transport within lipid bilayer; cell-substrate adhesion; cellular calcium ion homeostasis; formation of radial glial scaffolds; heterotypic cell-cell adhesion; negative regulation of neuron differentiation; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of Rho protein signal transduction; cell migration during sprouting angiogenesis; B cell differentiation; cell-cell adhesion mediated by integrin; calcium-independent cell-matrix adhesion; stress fiber formation; cellular defense response; response to activity; homophilic cell adhesion; blood coagulation; leukocyte tethering or rolling; leukocyte migration; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of cell migration; G1/S transition of mitotic cell cycle
Research Articles on ITGB1
Similar Products
Product Notes
The ITGB1 itgb1 (Catalog #AAA633220) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin, beta1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Immunoprecipitation, Flow Cytometry, Immunohistochemistry and Western Blot. Not suitable for use in Inhibition. Dilution: Immunoprecipitation: 2ug; lysate of 10e6 cell Flow Cytometry: 10ug/ml Immunohistochemistry (Frozen): 20ug/ml. As a result of the wide tissue distribution of VLA (b1), binding to diverse cell types is commonly observed. Western Blot: 1:1000 on 10ug C2C12 lysate. C2C12 lysates was resolved by electrophoresis, transferred to PDVF membrane and probed with I7661-39J. Proteins were visualized using an IgG (HRP) goat anti-rat and a chemiluminescent detection system. Inhibition: Does not block VLA integrin mediated cell adhesion to matrix proteins. Optimal dilution determined by the researcher. Researchers should empirically determine the suitability of the ITGB1 itgb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integrin, beta1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
