Rabbit anti-Human, Rat ADAM-17 (A Disintegrin And Metalloproteinase-17, TNF-a Converting Enzyme, TACE) Polyclonal Antibody | anti-ADAM-17 antibody

Anti-ADAM-17 (A Disintegrin And Metalloproteinase-17, TNF-a Converting Enzyme, TACE), Cytoplasmic Domain Antibody

Cat. Num. AAA4157464

• Reactivity: Human, Rat
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: ADAM-17 (A Disintegrin And Metalloproteinase-17; TNF-a Converting Enzyme; TACE); Polyclonal Antibody; Anti-ADAM-17 (A Disintegrin And Metalloproteinase-17; Cytoplasmic Domain Antibody; anti-ADAM-17 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody recognizes ADAM17, but does not react with other ADAMs.
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)

• Applicable Applications for anti-ADAM-17 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000 (when using colorimetric substrates such as BCIP/NBT) and 1:5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. Detects a band of approximately 110 kDa in reduced Western blots of conditioned media or cell lysates. (Predicted molecular weight: 93 kDa). Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.
• Immunogen: Synthetic peptide based on the cytoplasmic domain of full length human ADAM17.
• Cellular Localization: Membrane; Single Pass Type I Membrane Protein.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-ADAM-17 antibody

Description: The antibody is a polyclonal antibody made to the metalloproteinase ADAM-17. The antibody is made to a synthetic peptide based on the Cytoplasmic domain of full-length human ADAM-17. The antibody reacts with human, porcine and rat ADAM-17, and does not react with other ADAMs. The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of 0.01% Sodium Azide as preservative, and 50% glycerol as cryoprotectant. ADAM-17, also known as TNF-a Convertase, or TACE, was first cloned from human epithelial cells. The ADAMs proteins are structurally similar, with a signal sequence, metalloprotease domain (inactive in some ADAMs), disintegrin domain, cystein-rich domain, EGF-like repeat, type-I tyransmembrane and Cytoplasmic domain. A member of the metalloproteinase family containing disintegrin-like domains (ADAMs), ADAM-17 is known to process the TNF-a trimer from the membrane-attached precursor to the soluble form. ADAM-17 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active, cleaving TNF precursor as well as TNF p75 receptor, myeloid precursor protein, amyloid plaque protein, L-selectin and TGF-a, making ADAM17 a "sheddase." ADAM17 contains a cleavage site for prohormone convertases (furin), and the majority of ADAM17 is secreted as activated enzyme, cleaved at the furin site. ADAM17 is known to associate with MAD2 via the Cytoplasmic domain, implying cellular regulation pathways. ADAM-17 is expressed in a wide range of cells in culture. Three human ADAM17 sequences encode proteins with different Cytoplasmic domains, as is the case for other ADAMs proteases. The 824 amino acid sequence encodes a 92, 960 Dalton protein, the 807 AA sequence 91,003 Dalton, and the 694 AA sequence 78, 543 Dalton predicted MW. Only the full-length ADAM-17 contains the Cytoplasmic epitope for RP3ADAM-17. Glycosylation and other posttranslational modifications increase the apparent molecular weight on PAGE gels. Mouse and rat ADAM17 sequences are both 827 AA, predicting 93, 073 and 93, 017 Dalton proteins, respectively. RP3ADAM-17 recognizes a 110 kD band in reduced Western blots of conditioned media or cell lysates. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.

Relevance: ADAM17, also known as TNF alpha Convertase, or TACE, was first cloned from human epithelial cells. The ADAMs proteins are structurally similar, with a signal sequence, metalloprotease domain (inactive in some ADAMs), disintegrin domain, cystein rich domain, EGF like repeat, type I transmembrane and Cytoplasmic domain. A member of the metalloproteinase family containing disintegrin like domains (ADAMs), ADAM17 is known to process the TNF alpha trimer from the membrane attached precursor to the soluble form. ADAM17 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active, cleaving TNF precursor as well as TNF p75 receptor, myeloid precursor protein, amyloid plaque protein, L selectin and TGF alpha, making ADAM17 a "sheddase". ADAM17 contains a cleavage site for prohormone convertases (furin), and the majority of ADAM17 is secreted as activated enzyme, cleaved at the furin site. ADAM17 is known to associate with MAD2 via the Cytoplasmic domain, implying cellular regulation pathways. ADAM17 is expressed in a wide range of cells in culture. Three human ADAM17 sequences encode proteins with different cytoplasmic domains, as is the case for other ADAMs proteases. The 824 amino acid sequence encodes a 92,960 Dalton protein, the 807 AA sequence 91,003 Dalton, and the 694 AA sequence 78,543 Dalton predicted MW. Glycosylation and other post translational modifications increase the apparent molecular weight on PAGE gels. Mouse and rat ADAM17 sequences are both 827 AA, predicting 93,073 and 93,017 Dalton proteins, respectively. Reports have shown that ADAM17 expression in human breast tumor samples is an indicator of poor prognosis.