Rabbit N-WASP (Tyr-256) Polyclonal Antibody | anti-WASL antibody

Anti-N-WASP (Tyr-256), phospho-specific

Cat. Num. AAA472041

• Gene Names: WASL; NWASP; N-WASP; MGC48327; DKFZp779G0847
• Reactivity: Human, Rat, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified
• Synonyms: N-WASP (Tyr-256); Polyclonal Antibody; Anti-N-WASP (Tyr-256); phospho-specific; Neural Wiskott-Aldrich syndrome protein; WASL; anti-WASL antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: This antibody was cross-adsorbed to phosphotyrosine then affinity purified using phospho-N-WASP (Tyr-256) peptide (without carrier). The antibody detects a 65 kDa* protein corresponding to the molecular mass of phosphorylated N-WASP on SDS-PAGE immunoblots of A431 cells treated with pervanadate. A similar band is observed in pervanadate treated HeLa and endothelial cells. Weak bands are also observed at higher molecular weights after pervanadate treatment. These bands may be due to low cross-reactivity with phosphotyrosine.
• Purity/Purification: Affinity Purified
• Form/Format: Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-WASL antibody: ELISA, Western Blot
• Application Notes: ELISA: 1:20000; Western Blot: 1:10000
• Immunogen: Phospho-N-WASP (Tyr-256) synthetic peptide (coupled to BSA) corresponding to amino acid residues around tyrosine 256 of human N-WASP. The human WASP sequence has a two amino acid difference in the same region surrounding tyrosine 291.
• Preparation and Storage: Store at -20 degree C. Do not aliquot. Stable for 1 year.

Testing Data

Related Product Information for anti-WASL antibody

Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with higher homology in the functional regions of these proteins. Phosphorylation at serine and tyrosine residues regulates the activity of both proteins. WASP is tyrosine phosphorylated at tyrosine 291 after antigen receptor activation in B-cells and collagen stimulation of platelets. Phosphorylation of the analogous site in N-WASP (Tyr-256) stimulates its activity, reduces nuclear N-WASP, and is required for neurite extension.

References

• Baba, Y. et al. (1999) Blood 93:2003.
• Torres, E. & Rosen, M.K. (2003) Mol Cell 11:1215.
• Wu, X. et al. (2004) J Biol Chem 279(10):9565.

NCBI and Uniprot Product Information

• NCBI GI #: 51702526
• NCBI GeneID: 8976
• NCBI Accession #: NP_003932.3
• NCBI GenBank Nucleotide #: NM_003941.2
• UniProt Accession #: O00401; Q7Z746; Q68DE4
• Molecular Weight: 54,827 Da
• NCBI Official Full Name: neural Wiskott-Aldrich syndrome protein
• NCBI Official Synonym Full Names: Wiskott-Aldrich syndrome-like
• NCBI Official Symbol: WASL
• NCBI Official Synonym Symbols: NWASP; N-WASP; MGC48327; DKFZp779G0847
• NCBI Protein Information: neural Wiskott-Aldrich syndrome protein; OTTHUMP00000211994; Wiskott-Aldrich syndrome gene-like
• UniProt Protein Name: Neural Wiskott-Aldrich syndrome protein
• Protein Family: Neural Wiskott-Aldrich syndrome protein
• UniProt Gene Name: WASL
• UniProt Entry Name: WASL_HUMAN

NCBI Description

The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. The WASL gene product is a homolog of WAS protein, however, unlike the latter, it is ubiquitously expressed and shows highest expression in neural tissues. It has been shown to bind Cdc42 directly, and induce formation of long actin microspikes. [provided by RefSeq]

Uniprot Description

Function: Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. Ref.11

Subunit structure: Binds actin and the Arp2/3 complex. Interacts with CDC42. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with SNX9. Interacts with the WW domains of PRPF40A/FBP11

By similarity. Interacts with NOSTRIN. Interacts with Shigella flexneri protein icsA. The interaction with icsA enhances the affinity of WASL for Arp2/3, thus assembling a tight complex which has maximal activity in actin assembly. Interacts with E.coli EspF-like protein encoded on prophage U. Identified in a complex containing at least WASL, BAIAP2L1 and E.coli EspF-like protein encoded on prophage U. Ref.4 Ref.5 Ref.6 Ref.11

Subcellular location: Cytoplasm › cytoskeleton

By similarity. Nucleus

By similarity. Note: Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated

Sequence similarities: Contains 1 CRIB domain.Contains 1 WH1 domain.Contains 2 WH2 domains.