Rabbit SOX-9 Antibody | anti-SOX-9 antibody

Rabbit anti SOX-9 antibody

Cat. Num. AAA462136

• Reactivity: Human, Rat, Mouse, Chicken, Bovine, Canine
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
• Synonyms: SOX-9; Rabbit anti SOX-9 antibody; Rabbit anti-SOX-9; Sry-related high-mobility group (HMG) box 9; anti-SOX-9 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse, Chicken, Bovine, Canine
• Specificity: This antibody recognizes ~56 kDa of human SOX-9 protein. It also reacts with mouse and rat. The other species are not tested.
• Purity/Purification: The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
• Form/Format: This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Concentration: Size: 100 ug/200 ul (varies by lot)

• Applicable Applications for anti-SOX-9 antibody: ELISA, Western Blot, Immunohistochemistry
• Application Notes: Western Blot: 0.1-1 ug/ml; ELISA: 0.01-0.1 ug/ml; Immunoprecipitation: 2-5 ug/ml; Immunohistochemistry: 2-5 ug/ml
• Antigen Preparation: A synthetic peptide derived from internal sequence of Human SOX-9 protein. This sequence is identical among human, rat, mouse, bovine, chicken, and dog.
• Positive Control: LNCaP prostate Ca Cell
• Cellular Location: Nuclear
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Testing Data

Related Product Information for anti-SOX-9 antibody

The SOX9 is a member of the SOX [Sry-related high-mobility group (HMG) box] family of HMG DNA-binding domain transcription factors and is required for the development and differentiation of multiple cell lineages. SOX9 is expressed in primary prostate cancers in vivo, at a higher frequency in recurrent prostate cancer and in prostate cancer cell lines (LNCaP, CWR22, PC3, and DU145). It has been reported that the cAMP/PKA stimulation induces SOX-9 nuclear translocation via facilitating its nuclear import by the phosphorylation of SOX-9 at Ser64 and Ser181. SOX9 in prostate basal cells supports the development and maintenance of the luminal epithelium and that a subset of prostate cancer cells may escape basal cell requirements through SOX9 expression.

References

Hongyun Wang et al. SOX9 Is Expressed in Normal Prostate Basal Cells and Regulates Androgen Receptor Expression in Prostate Cancer Cells. Cancer Research 67, 528-536, January 15, 2007.

NCBI and Uniprot Product Information

• NCBI GI #: 938234
• UniProt Accession #: P41225; P35714; Q5JWI3; Q9NP49
• Molecular Weight: 56 kDa
• NCBI Official Full Name: SOX-9
• UniProt Protein Name: Transcription factor SOX-3
• UniProt Gene Name: SOX3
• UniProt Entry Name: SOX3_HUMAN

NCBI Description

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. Mutations in this gene have been associated with X-linked mental retardation with growth hormone deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Transcription factor required during the formation of the hypothalamo-pituitary axis. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Required also within the pharyngeal epithelia for craniofacial morphogenesis. Controls a genetic switch in male development. Is necessary for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells

By similarity. Ref.6

Subunit structure: Interacts with SOX2 and FGFR1

Subcellular location: Nucleus.

Involvement in disease: Defects in SOX3 are a cause of panhypopituitarism X-linked (PHPX) [

MIM:312000]. Affected individuals have absent infundibulum, anterior pituitary hypoplasia, and ectopic posterior pituitary. Ref.8Defects in SOX3 are the cause of mental retardation X-linked with isolated growth hormone deficiency (MRXGH) [

MIM:300123]. Ref.7Defects in SOX3 are the cause of 46,XX sex reversal type 3 (SRXX3) [

MIM:300833]. A condition in which male gonads develop in a genetic female (female to male sex reversal). Note=Copy number variations (CNV) encompassing or in close proximity to SOX3 are responsible for XX male reversal. These variations include two duplications of approximately 123 kb and 85 kb, the former of which spans the entire SOX3 gene; a 343 kb deletion immediately upstream of SOX3 that is probably responsible of altered regulation (and not increased dosage) of SOX3; a large (approximately 6 Mb) duplication that encompasses SOX3 and at least 18 additional distally located genes. Its proximal breakpoint falls within the SOX3 regulatory region. This large rearrangement has been found in a patient with XX male reversal and a complex phenotype that also includes a scrotal hypoplasia, microcephaly, developmental delay, and growth retardation. Ref.6

Sequence similarities: Contains 1 HMG box DNA-binding domain.

Caution: Was originally (Ref.5) termed SOX-9.