Human ATP Binding Cassette Transporter A3 (ABCA3) ELISA Kit | ABCA3 elisa kit

Human ATP Binding Cassette Transporter A3 (ABCA3) ELISA Kit

Cat. Num. AAA451927

• Gene Names: ABCA3; ABC3; ABC-C; SMDP3; LBM180; EST111653
• Reactivity: Human
• Synonyms: ATP Binding Cassette Transporter A3 (ABCA3); Human ATP Binding Cassette Transporter A3 (ABCA3) ELISA Kit; ABC-A3; ABC-C; ABC3; ABC-C transporter; ABCA3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ABCA3. No significant cross-reactivity or interference between ABCA3 and analogues was observed.
• Sequence Length: 1704

• Samples: Human tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.1-5000pg/mL
• Sensitivity: < 29pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ABCA3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ABCA3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ABCA3 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ABCA3 in human tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ABCA3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ABCA3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ABCA3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ABCA3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 116734710
• NCBI GeneID: 21
• NCBI Accession #: NP_001080.2
• NCBI GenBank Nucleotide #: NM_001089.2
• UniProt Accession #: Q99758; Q54A95; Q6P5P9; Q92473; B2RU09
• Molecular Weight: 24,002 Da
• NCBI Official Full Name: ATP-binding cassette sub-family A member 3
• NCBI Official Synonym Full Names: ATP binding cassette subfamily A member 3
• NCBI Official Symbol: ABCA3
• NCBI Official Synonym Symbols: ABC3; ABC-C; SMDP3; LBM180; EST111653
• NCBI Protein Information: ATP-binding cassette sub-family A member 3
• UniProt Protein Name: ATP-binding cassette sub-family A member 3
• Protein Family: ABC transporter A family
• UniProt Gene Name: ABCA3
• UniProt Synonym Gene Names: ABC3; ATP-binding cassette 3

NCBI Description

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. The full transporter encoded by this gene may be involved in development of resistance to xenobiotics and engulfment during programmed cell death. [provided by RefSeq, Jul 2008]

Uniprot Description

Plays an important role in the formation of pulmonary surfactant, probably by transporting lipids such as cholesterol.