SDS-Page
(SDS-PAGE Analysis Purified EIF4A2 Mouse Monoclonal Antibody (PCRP-EIF4A2-2B5). Confirmation of Purity and Integrity of Antibody. )
Mouse EIF4A2 (Eukaryotic Initiation Factor 4A-II) Monoclonal Antibody | anti-EIF4A2 antibody
EIF4A2 (Eukaryotic Initiation Factor 4A-II) Mouse Monoclonal Antibody [Clone PCRP-EIF4A2-2B5]
Gene Names
EIF4A2; DDX2B; EIF4A; EIF4F; BM-010; eIF4A-II; eIF-4A-II
Reactivity
HumanPredicted to work in Chick, Mouse, Rat, Xenopus and Zebrafish.
Applications
ELISA
Synonyms
EIF4A2 (Eukaryotic Initiation Factor 4A-II); Monoclonal Antibody; EIF4A2 (Eukaryotic Initiation Factor 4A-II) Mouse Monoclonal Antibody [Clone PCRP-EIF4A2-2B5]; ATP dependent RNA helicase eIF4A 2; BM-010; DDX2B; EIF4F; eukaryotic translation initiation factor 4A isoform 2; N-terminally processed; anti-EIF4A2 antibody
Host
Mouse
Reactivity
Human
Predicted to work in Chick, Mouse, Rat, Xenopus and Zebrafish.
Predicted to work in Chick, Mouse, Rat, Xenopus and Zebrafish.
Clonality
Monoclonal
Isotype
Mouse/IgG2a
Clone Number
PCRP-EIF4A2-2B5
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
Purified Ab with BSA and Azide at 200ug/ml
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
Applicable Applications for anti-EIF4A2 antibody
ELISA (EIA)
Application Notes
ELISA: For coating, order Ab without BSA)
Immunogen
Recombinant full-length human EIF4A2 protein
Cellular Localization
Nucleus
Positive Control
HeLa cells.
Hu-chromosome Location
3q27.3
Unigene
518475 & 599481
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
SDS-Page
(SDS-PAGE Analysis Purified EIF4A2 Mouse Monoclonal Antibody (PCRP-EIF4A2-2B5). Confirmation of Purity and Integrity of Antibody. )
SDS-Page
(SDS-PAGE Analysis Purified EIF4A2 Mouse Monoclonal Antibody (PCRP-EIF4A2-2B5). Confirmation of Purity and Integrity of Antibody. )
Related Product Information for anti-EIF4A2 antibody
Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Mammalian eukaryotic translation initiation factor 4F (eIF4F) is a protein complex that contains eIF4A, eIF4E and eIF4G, binds mRNA at a5'-cap motif and recruits the 43S ribosomal preinitiation complex to the eligible transcript. Along with eIF4B, the eIF4F complex mediates the unwinding of mRNA secondary structure to facilitate ribosome association. eIF4E specifically interacts with the 5' cap, eIF4A (I, II) are bidirectional RNA helicases, and eIF4G (I, II) are scaffolding proteins which coordinate eIF4E, eIF4A, eIF3and the 40S ribosome. Human eIF4AI (eIF4A, DDX2A) is a 406 amino acid protein that is 92. 7% homologous to mouse eIF4AI. The promoter region of human eIF4A1 contains TATA and CAAT motifs and consensus binding sites to Sp1 and AP2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
407
NCBI Official Full Name
Eukaryotic initiation factor 4A-II
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4A2
NCBI Official Symbol
EIF4A2
NCBI Official Synonym Symbols
DDX2B; EIF4A; EIF4F; BM-010; eIF4A-II; eIF-4A-II
NCBI Protein Information
eukaryotic initiation factor 4A-II; ATP-dependent RNA helicase eIF4A-2
UniProt Protein Name
Eukaryotic initiation factor 4A-II
Protein Family
UniProt Gene Name
EIF4A2
UniProt Synonym Gene Names
DDX2B; EIF4F; eIF-4A-II; eIF4A-II
UniProt Entry Name
IF4A2_HUMAN
Uniprot Description
EIF4A2: ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. Belongs to the DEAD box helicase family. eIF4A subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA-binding; Translation; Translation initiation; Helicase; EC 3.6.4.13
Chromosomal Location of Human Ortholog: 3q28
Cellular Component: perinuclear region of cytoplasm; eukaryotic translation initiation factor 4F complex; cytosol
Molecular Function: protein binding; translation initiation factor activity; ATPase activity; helicase activity; ATP binding
Biological Process: poly(A) tail shortening; cellular protein metabolic process; translation; viral reproduction; cytokine and chemokine mediated signaling pathway; translational initiation; gene expression; regulation of translational initiation; mRNA catabolic process, deadenylation-dependent decay
Research Articles on EIF4A2
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Product Notes
The EIF4A2 eif4a2 (Catalog #AAA4381846) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EIF4A2 (Eukaryotic Initiation Factor 4A-II) Mouse Monoclonal Antibody [Clone PCRP-EIF4A2-2B5] reacts with Human Predicted to work in Chick, Mouse, Rat, Xenopus and Zebrafish. and may cross-react with other species as described in the data sheet. AAA Biotech's EIF4A2 (Eukaryotic Initiation Factor 4A-II) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). ELISA: For coating, order Ab without BSA). Researchers should empirically determine the suitability of the EIF4A2 eif4a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF4A2 (Eukaryotic Initiation Factor 4A-II), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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