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Western Blot (WB) ((1ug/ml) staining of Human (A) and Mouse (B) Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat ETEA/UBXD8 Polyclonal Antibody | anti-FAF2 antibody

Goat anti-ETEA/UBXD8 Antibody

Gene Names
FAF2; ETEA; UBXD8; UBXN3B
Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
ETEA/UBXD8; Polyclonal Antibody; Goat anti-ETEA/UBXD8 Antibody; FAF2; ETEA; UBXD8; KIAA0887; expressed in T-cells and eosinophils in atopic dermatitis; UBX domain containing 8; Fas associated factor family member 2; UBXN3B; UBX domain protein 3B; FAF2 antibody; ETEA antibody; UBXD8 antibody; KIAA0887 antibody; expressed in T-cells and eosinophils in atopic dermatitis antibody; UBX domain containing 8 antibody; Fas associated factor family member 2 antibody; UBXN3B antibody; UBX domain protein 3B antibody; ETEA / UBXD8; anti-FAF2 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
HTEVLFVQDLTDE
Sequence Length
445
Applicable Applications for anti-FAF2 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 55kDa band observed in lysates of Human and Mouse Thymus and of cell line Jurkat (calculated MW of 52.6kDa according to NP_055428.1). Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence C-HTEVLFVQDLTDE, from the C Terminus of the protein sequence according to NP_055428.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of Human (A) and Mouse (B) Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((1ug/ml) staining of Human (A) and Mouse (B) Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB)

(Staining (0.3ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

Western Blot (WB) (Staining (0.3ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52,623 Da
NCBI Official Full Name
FAS-associated factor 2
NCBI Official Synonym Full Names
Fas associated factor family member 2
NCBI Official Symbol
FAF2
NCBI Official Synonym Symbols
ETEA; UBXD8; UBXN3B
NCBI Protein Information
FAS-associated factor 2
UniProt Protein Name
FAS-associated factor 2
Protein Family
UniProt Gene Name
FAF2
UniProt Synonym Gene Names
ETEA; KIAA0887; UBXD8; UBXN3B
UniProt Entry Name
FAF2_HUMAN

NCBI Description

The protein encoded by this gene is highly expressed in peripheral blood of patients with atopic dermatitis (AD), compared to normal individuals. It may play a role in regulating the resistance to apoptosis that is observed in T cells and eosinophils of AD patients. [provided by RefSeq, Jul 2008]

Uniprot Description

ETEA: May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: endoplasmic reticulum; lipid particle

Molecular Function: lipase inhibitor activity; protein binding; ubiquitin binding; ubiquitin protein ligase binding

Biological Process: ER-associated protein catabolic process; negative regulation of catalytic activity; response to unfolded protein; retrograde protein transport, ER to cytosol

Research Articles on FAF2

Similar Products

Product Notes

The FAF2 faf2 (Catalog #AAA421122) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ETEA/UBXD8 Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's ETEA/UBXD8 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 55kDa band observed in lysates of Human and Mouse Thymus and of cell line Jurkat (calculated MW of 52.6kDa according to NP_055428.1). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the FAF2 faf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: HTEVLFVQDL TDE. It is sometimes possible for the material contained within the vial of "ETEA/UBXD8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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