LYVE1 recombinant protein

LYVE1 Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8122510

• Gene Names: Lyve1; Xlkd1; Lyve-1; Crsbp-1; 1200012G08Rik
• Purity: >85% as determined by SDS-PAGE
• Synonyms: LYVE1; LYVE1 Protein; Mouse; Recombinant (hFc Tag); Mouse LYVE1/LYVE-1 Protein (Fc Tag); 1200012G08Rik Protein; Crsbp-1 Protein; Lyve-1 Protein; Xlkd1 Protein; lymphatic vessel endothelial hyaluronan receptor 1; LYVE1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly228

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 24
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse LYVE1 (Q8BHC0)(Met1-Gly228) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for LYVE1 recombinant protein

Background: LYVE1, also known as LYVE-1, is a type I integral membrane glycoprotein. It contains 1 Link domain and is mainly expressed in endothelial cells lining lymphatic vessels. LYVE1 acts as a receptor and binds to both soluble and immobilized hyaluronan. It may function in lymphatic hyaluronan transport and have a role in tumor metastasis. LYVE1 also is a cell surface receptor on lymphatic endothelial cells that can be used as a lymphatic endothelial cell marker, and sort these cells for experimental purposes. It also functions as a ligand-specific transporter trafficking between intracellular organelles and the plasma membrane. It plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding. It may act as a hyaluronan transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.

References

Jackson DG. (2003) The lymphatics revisited: new perspectives from the hyaluronan receptor LYVE-1. Trends Cardiovasc Med. 13(1):1-7.Banerji S, et al. (1999) LYVE-1, a new homologue of the CD44 glycoprotein, is a lymph-specific receptor for hyaluronan. J Cell Biol. 144(4):789-801.Mouta Carreira C, et al. (2001) LYVE-1 is not restricted to the lymph vessels: expression in normal liver blood sinusoids and down-regulation in human liver cancer and cirrhosis. Cancer Res. 61(22): 8079-84.Cursiefen C, et al. (2002) Lymphatic vessels in vascularized human corneas: immunohistochemical investigation using LYVE-1 and podoplanin. Invest Ophthalmol Vis Sci. 43(7):2127-35.

NCBI and Uniprot Product Information

• NCBI GI #: 31560771
• NCBI GeneID: 114332
• NCBI Accession #: NP_444477.2
• NCBI GenBank Nucleotide #: NM_053247.4
• UniProt Accession #: Q8BHC0; Q3TUC1; Q99NE4
• Molecular Weight: 34,574 Da
• NCBI Official Full Name: lymphatic vessel endothelial hyaluronic acid receptor 1
• NCBI Official Synonym Full Names: lymphatic vessel endothelial hyaluronan receptor 1
• NCBI Official Symbol: Lyve1
• NCBI Official Synonym Symbols: Xlkd1; Lyve-1; Crsbp-1; 1200012G08Rik
• NCBI Protein Information: lymphatic vessel endothelial hyaluronic acid receptor 1; cell surface retention sequence binding protein-1; cell surface retention sequence-binding protein 1; extra cellular link domain-containing 1; extracellular link domain-containing protein 1; lymphat
• UniProt Protein Name: Lymphatic vessel endothelial hyaluronic acid receptor 1
• Protein Family: Lymphatic vessel endothelial hyaluronic acid receptor
• UniProt Gene Name: Lyve1
• UniProt Synonym Gene Names: Crsbp1; Xlkd1; LYVE-1; CRSBP-1
• UniProt Entry Name: LYVE1_MOUSE

Uniprot Description

XLKD1: Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as an hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis

Cellular Component: membrane; integral to membrane; plasma membrane

Molecular Function: transmembrane receptor activity; hyaluronic acid binding

Biological Process: glycosaminoglycan catabolic process; transport; cell adhesion; signal transduction