Western Blot (WB)
(Western Blot: Sample: Recombinant ERb, Mouse.)
Rabbit anti-Mouse Estrogen Receptor Beta (ERb) Polyclonal Antibody | anti-ERb antibody
Polyclonal Antibody to Estrogen Receptor Beta (ERb)
MGHHHHHHSGSEF-MEIKNSPSSL TSPASYNCSQ SILPLEHGPI YIPSSYVESR HEYSAMTFYS PAVMNYSVPS STGNLEGGPV RQTASPNVLW PTSGHLSPLA THCQSSLLYA EPQKSPWCEA RSLEHTLPVN RETLKRKLGG SGCASPVTSP SAKRDAHFCA VCSDYASGYH YGVWSCEGCK AFFKRSIQGH NDYICPATNQ CTIDKNRRKS CQACRLRKCY EVGMVKCGSR RERCGYRIVR RQRSASEQVH CLNKAKRTSG HTPRVKELLL NSLSPEQLVL TLLEAEPPNV LVSRPSMPFT EASMMMSLTK LADKELVHMI GWAKKIPGFV ELSLLDQVRL LESCWMEVLM VGLMWRSIDH PGKLIFAPDL VLDRDEGKCV EGILEIFDML LATTARFREL KLQHKEYLCV KAMILLNSSM YPLATASQEA ESSRKLTHLL NAVTDALVWV ISKSGISSQQ QSVRLANLLM LLSHVRHISN KGMEHLLSMK CKNVVPVYDL LLEMLNAHTL RGYKSSISGS ECCSTEDSKS KEGSQNLQSQ
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Recombinant ERb, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant ERb, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Ovary tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Ovary tissue))
NCBI and Uniprot Product Information
Uniprot Description
ER-beta: a nuclear hormone receptor and transcription factor. Binds and activated by estrogen. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Binds estrogens with an affinity similar to that of ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Eight alternatively-spliced isoforms have been described. Isoform beta-cx lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.
Protein type: DNA-binding; Nuclear receptor
Chromosomal Location of Human Ortholog: 12 C3|12 33.52 cM
Cellular Component: cell soma; cilium; cytoplasm; mitochondrion; neuron projection; nucleus; perikaryon; perinuclear region of cytoplasm; plasma membrane
Molecular Function: DNA binding; DNA binding transcription factor activity; drug binding; enzyme binding; estradiol binding; estrogen receptor activity; estrogen response element binding; hormone binding; identical protein binding; lipid binding; metal ion binding; peroxisome proliferator activated receptor binding; protein binding; RNA polymerase II transcription factor activity, estrogen-activated sequence-specific DNA binding; sequence-specific DNA binding; steroid binding; steroid hormone binding; steroid hormone receptor activity; zinc ion binding
Biological Process: behavioral fear response; brain development; cellular response to estradiol stimulus; hormone-mediated apoptotic signaling pathway; intracellular estrogen receptor signaling pathway; learning and/or memory; negative regulation of behavior; negative regulation of cell proliferation; negative regulation of epithelial cell proliferation; negative regulation of feeding behavior; negative regulation of neuron death; negative regulation of reactive oxygen species metabolic process; negative regulation of smooth muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; neuron migration; ovarian follicle development; positive regulation of apoptosis; positive regulation of blood vessel diameter; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of ERK1 and ERK2 cascade; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of cell proliferation; regulation of neuron apoptosis; regulation of transcription, DNA-templated; response to testosterone stimulus; Sertoli cell proliferation; steroid hormone mediated signaling; steroid hormone receptor signaling pathway; transcription, DNA-dependent; uterus development; vagina development
Research Articles on ERb
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Product Notes
The ERb esr2 (Catalog #AAA2005331) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Estrogen Receptor Beta (ERb) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Estrogen Receptor Beta (ERb) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ERb esr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with a His-tag and its sequence is listed below. MGHHHHHHSG SEF-MEIKNS PSSL TSPASYNCSQ SILPLEHGPI YIPSSYVESR HEYSAMTFYS PAVMNYSVPS STGNLEGGPV RQTASPNVLW PTSGHLSPLA THCQSSLLYA EPQKSPWCEA RSLEHTLPVN RETLKRKLGG SGCASPVTSP SAKRDAHFCA VCSDYASGYH YGVWSCEGCK AFFKRSIQGH NDYICPATNQ CTIDKNRRKS CQACRLRKCY EVGMVKCGSR RERCGYRIVR RQRSASEQVH CLNKAKRTSG HTPRVKELLL NSLSPEQLVL TLLEAEPPNV LVSRPSMPFT EASMMMSLTK LADKELVHMI GWAKKIPGFV ELSLLDQVRL LESCWMEVLM VGLMWRSIDH PGKLIFAPDL VLDRDEGKCV EGILEIFDML LATTARFREL KLQHKEYLCV KAMILLNSSM YPLATASQEA ESSRKLTHLL NAVTDALVWV ISKSGISSQQ QSVRLANLLM LLSHVRHISN KGMEHLLSMK CKNVVPVYDL LLEMLNAHTL RGYKSSISGS ECCSTEDSKS KEGSQNLQSQ. It is sometimes possible for the material contained within the vial of "Estrogen Receptor Beta (ERb), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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