Human Estrogen Receptor Beta (ERb) CLIA Kit | ERb clia kit

Human Estrogen Receptor Beta (ERb) CLIA Kit

Cat. Num. AAA2000410

• Gene Names: Esr2; ER[b]; Estrb; ERbeta
• Reactivity: Human
• Synonyms: Estrogen Receptor Beta (ERb); Human Estrogen Receptor Beta (ERb) CLIA Kit; ER-B; ESRB; ESTRB; NR3A2; NR3-A2; ESR2; Estrogen Receptor 2; Nuclear Receptor Subfamily 3; Group A; Member 2; ERb clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Estrogen Receptor Beta (ERb).; No significant cross-reactivity or interference between Estrogen Receptor Beta (ERb) and analogues was observed.
• Sequence Length: 567

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 27.4-20,000pg/mL
• Sensitivity: < 10.1pg/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Estrogen Receptor Beta (ERb) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Estrogen Receptor Beta (ERb) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ERb clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Estrogen Receptor Beta (ERb). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Estrogen Receptor Beta (ERb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Estrogen Receptor Beta (ERb) level in the sample or standard.;

Product Categories/Family for ERb clia kit

Endocrinology; Reproductive science; Hormone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 46877096
• NCBI GeneID: 13983
• NCBI Accession #: NP_997590.1
• NCBI GenBank Nucleotide #: NM_207707.1
• UniProt Accession #: O08537; O35635; O70519; Q8BG65; Q91Z86; B2RUC6; E9QKX7
• Molecular Weight: 42,107 Da
• NCBI Official Full Name: estrogen receptor beta isoform 1
• NCBI Official Synonym Full Names: estrogen receptor 2 (beta)
• NCBI Official Symbol: Esr2
• NCBI Official Synonym Symbols: ER[b]; Estrb; ERbeta
• NCBI Protein Information: estrogen receptor beta
• UniProt Protein Name: Estrogen receptor beta
• UniProt Gene Name: Esr2
• UniProt Synonym Gene Names: Estrb; Nr3a2; ER-beta
• UniProt Entry Name: ESR2_MOUSE

Uniprot Description

ER-beta: a nuclear hormone receptor and transcription factor. Binds and activated by estrogen. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Binds estrogens with an affinity similar to that of ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Eight alternatively-spliced isoforms have been described. Isoform beta-cx lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.

Protein type: Nuclear receptor; DNA-binding

Cellular Component: neuron projection; cell soma; mitochondrion; perinuclear region of cytoplasm; cytoplasm; plasma membrane; perikaryon; nucleus; cilium

Molecular Function: ligand-dependent nuclear receptor activity; peroxisome proliferator activated receptor binding; estrogen receptor activity; hormone binding; zinc ion binding; metal ion binding; estrogen response element binding; drug binding; steroid binding; protein binding; enzyme binding; DNA binding; sequence-specific DNA binding; steroid hormone receptor activity; lipid binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; behavioral fear response; estrogen receptor signaling pathway; positive regulation of apoptosis; induction of apoptosis by hormones; negative regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; neuron migration; negative regulation of transcription from RNA polymerase II promoter; uterus development; negative regulation of cell proliferation; learning and/or memory; ovarian follicle development; regulation of transcription, DNA-dependent; positive regulation of epidermal growth factor receptor signaling pathway; regulation of neuron apoptosis; negative regulation of epithelial cell proliferation; steroid hormone receptor signaling pathway; transcription, DNA-dependent; vagina development; Sertoli cell proliferation; response to testosterone stimulus; regulation of cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; brain development; negative regulation of behavior