Typical Testing Data/Standard Curve (for reference only)
Human ERbeta (Estrogen Receptor Beta) CLIA Kit | ERb clia kit
Human ERbeta (Estrogen Receptor Beta) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to ERbeta. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ERbeta and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ERbeta, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of ERbeta. You can calculate the concentration of ERbeta in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
ER-beta: a nuclear hormone receptor and transcription factor. Binds and activated by estrogen. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Binds estrogens with an affinity similar to that of ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Eight alternatively-spliced isoforms have been described. Isoform beta-cx lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.
Protein type: Nuclear receptor; DNA-binding
Cellular Component: neuron projection; mitochondrion; cell soma; perinuclear region of cytoplasm; cytoplasm; plasma membrane; perikaryon; nucleus; cilium
Molecular Function: peroxisome proliferator activated receptor binding; ligand-dependent nuclear receptor activity; estrogen receptor activity; hormone binding; zinc ion binding; metal ion binding; estrogen response element binding; drug binding; steroid binding; protein binding; enzyme binding; DNA binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription factor activity; lipid binding
Biological Process: transcription from RNA polymerase II promoter; behavioral fear response; estrogen receptor signaling pathway; induction of apoptosis by hormones; negative regulation of smooth muscle cell proliferation; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; neuron migration; negative regulation of transcription from RNA polymerase II promoter; positive regulation of vasodilation; uterus development; negative regulation of cell proliferation; learning and/or memory; regulation of transcription, DNA-dependent; ovarian follicle development; positive regulation of epidermal growth factor receptor signaling pathway; regulation of neuron apoptosis; negative regulation of epithelial cell proliferation; steroid hormone receptor signaling pathway; transcription, DNA-dependent; vagina development; Sertoli cell proliferation; response to testosterone stimulus; regulation of cell proliferation; positive regulation of transcription factor activity; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; brain development; negative regulation of behavior
Research Articles on ERb
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Product Notes
The Human ERb esr2 (Catalog #AAA2531725) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2531725 CLIA Kit recognizes Human ERb. It is sometimes possible for the material contained within the vial of "ERbeta (Estrogen Receptor Beta), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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