Estrogen Receptor Beta (ERb) Recombinant Protein | ERb recombinant protein

Recombinant Estrogen Receptor Beta (ERb)

Cat. Num. AAA2012018

• Gene Names: Esr2; ER[b]; Estrb; ERbeta
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Estrogen Receptor Beta (ERb); Recombinant Estrogen Receptor Beta (ERb); ERb recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-MEIKNSPSSL TSPASYNCSQ SILPLEHGPI YIPSSYVESR HEYSAMTFYS PAVMNYSVPS STGNLEGGPV RQTASPNVLW PTSGHLSPLA THCQSSLLYA EPQKSPWCEA RSLEHTLPVN RETLKRKLGG SGCASPVTSP SAKRDAHFCA VCSDYASGYH YGVWSCEGCK AFFKRSIQGH NDYICPATNQ CTIDKNRRKS CQACRLRKCY EVGMVKCGSR RERCGYRIVR RQRSASEQVH CLNKAKRTSG HTPRVKELLL NSLSPEQLVL TLLEAEPPNV LVSRPSMPFT EASMMMSLTK LADKELVHMI GWAKKIPGFV ELSLLDQVRL LESCWMEVLM VGLMWRSIDH PGKLIFAPDL VLDRDEGKCV EGILEIFDML LATTARFREL KLQHKEYLCV KAMILLNSSM YPLATASQEA ESSRKLTHLL NAVTDALVWV ISKSGISSQQ QSVRLANLLM LLSHVRHISN KGMEHLLSMK CKNVVPVYDL LLEMLNAHTL RGYKSSISGS ECCSTEDSKS KEGSQNLQSQ
• Sequence Length: 549

• Applicable Applications for ERb recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Met1~Gln530 (Accession # O08537) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 46877094
• NCBI GeneID: 13983
• NCBI Accession #: NP_034287.3
• NCBI GenBank Nucleotide #: NM_010157.3
• UniProt Accession #: O08537; O35635; O70519; Q8BG65; Q91Z86; B2RUC6; E9QKX7
• Molecular Weight: 60.6kDa
• NCBI Official Full Name: estrogen receptor beta isoform 2
• NCBI Official Synonym Full Names: estrogen receptor 2 (beta)
• NCBI Official Symbol: Esr2
• NCBI Official Synonym Symbols: ER[b]; Estrb; ERbeta
• NCBI Protein Information: estrogen receptor beta; ER beta; ER-beta; oestrogen receptor beta; nuclear receptor subfamily 3 group A member 2
• UniProt Protein Name: Estrogen receptor beta
• UniProt Gene Name: Esr2
• UniProt Synonym Gene Names: Estrb; Nr3a2; ER-beta
• UniProt Entry Name: ESR2_MOUSE

Uniprot Description

ER-beta: a nuclear hormone receptor and transcription factor. Binds and activated by estrogen. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Binds estrogens with an affinity similar to that of ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Eight alternatively-spliced isoforms have been described. Isoform beta-cx lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.

Protein type: Nuclear receptor; DNA-binding

Cellular Component: neuron projection; mitochondrion; cell soma; perinuclear region of cytoplasm; cytoplasm; plasma membrane; perikaryon; nucleus; cilium

Molecular Function: ligand-dependent nuclear receptor activity; peroxisome proliferator activated receptor binding; estrogen receptor activity; hormone binding; zinc ion binding; metal ion binding; estrogen response element binding; drug binding; steroid binding; protein binding; enzyme binding; DNA binding; sequence-specific DNA binding; steroid hormone receptor activity; lipid binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; behavioral fear response; estrogen receptor signaling pathway; induction of apoptosis by hormones; positive regulation of apoptosis; negative regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; neuron migration; negative regulation of transcription from RNA polymerase II promoter; uterus development; negative regulation of cell proliferation; learning and/or memory; regulation of transcription, DNA-dependent; ovarian follicle development; positive regulation of epidermal growth factor receptor signaling pathway; regulation of neuron apoptosis; negative regulation of epithelial cell proliferation; steroid hormone receptor signaling pathway; transcription, DNA-dependent; vagina development; Sertoli cell proliferation; response to testosterone stimulus; regulation of cell proliferation; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; brain development; negative regulation of behavior