Goat ERV3 Polyclonal Antibody | anti-ERV3 antibody

Goat anti-ERV3 Antibody

Cat. Num. AAA422488

• Gene Names: ERV3-1; ERV3; ERVR; envR; ERV-R; HERVR; HERV-R
• Reactivity: Expected from sequence similarity: Human
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: ERV3; Polyclonal Antibody; Goat anti-ERV3 Antibody; endogenous retroviral sequence 3 (includes zinc finger protein H-plk/HPF9); ERV-R; H-PLK; HERV-R; ERV-3 envelope protein; endogenous retroviral sequence 3; envelope polyprotein; endogenous retroviral sequence 3 antibody; endogenous retroviral sequence 3 (includes zinc finger protein H-plk/HPF9) antibody; envelope polyprotein antibody; ERV-3 envelope protein antibody; ERV-R antibody; HERV-R antibody; H-PLK antibody; ERV3 antibody; anti-ERV3 antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: PQDNFTLTASS
• Sequence Length: 604

• Applicable Applications for anti-ERV3 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 4000.; Western Blot: Preliminary experiments gave an approx 30kDa band in Human Placenta lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 67.9kDa according to NP_001007254.2. The 30kDa band was successfully blocked by incubation with the immunizing peptide.
• Immunogen: Peptide with sequence C-PQDNFTLTASS, from the internal region of the protein sequence according to NP_001007254.2.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 145651814
• NCBI GeneID: 2086
• NCBI Accession #: NP_001007254.2
• NCBI GenBank Nucleotide #: NM_001007253.3
• Molecular Weight: 67,942 Da
• NCBI Official Full Name: endogenous retrovirus group 3 member 1 Env polyprotein
• NCBI Official Synonym Full Names: endogenous retrovirus group 3 member 1
• NCBI Official Symbol: ERV3-1
• NCBI Official Synonym Symbols: ERV3; ERVR; envR; ERV-R; HERVR; HERV-R
• NCBI Protein Information: endogenous retrovirus group 3 member 1 Env polyprotein
• UniProt Protein Name: Endogenous retrovirus group 3 member 1 Env polyprotein
• UniProt Gene Name: ERV3-1
• UniProt Synonym Gene Names: ERV3; ERV3 envelope protein; ERV-R envelope protein; SU; TM
• UniProt Entry Name: ENR1_HUMAN

NCBI Description

The human genome includes many retroelements including the human endogenous retroviruses (HERVs). ERV3, one of the most studied HERVs, is thought to have integrated 30 to 40 million years ago and is present in higher primates with the exception of gorillas. Taken together, the observation of genome conservation, the detection of transcript expression, and the presence of conserved ORFs is circumstantial evidence for a functional role. A functional role is also suggested by the observation that downregulation of ERV3 is reported in choriocarcinoma. [provided by RefSeq, Jul 2008]

Uniprot Description

ERV3: Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. This endogenous envelope protein has lost its fusogenic properties. It can inhibit cell growth through decrease expression of cyclin B1 and increased expression of p21 in vitro. Belongs to the gamma type-C retroviral envelope protein family. HERV class-I R env subfamily.

Chromosomal Location of Human Ortholog: 7q11.2

Cellular Component: viral envelope