Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse CCL2 Standard Curve.)
Mouse CCL2 ELISA Kit | CCL2 elisa kit
Mouse CCL2 ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse CCL2 Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse CCL2 Standard Curve.)
Background: CCL2 is a small cytokine belonging to the CC chemokine family that is also known as monocyte chemotactic protein-1 (MCP-1). This chemokine is produced predominantly by macrophages and endothelial cells and is a potent chemotactic factor for monocytes as a protein precursor containing signal peptide of 23 amino acids and a mature peptide of 76 amino acids. It is a monomeric polypeptide, with a molecular weight of approximately 13kDa.CCL2 recruits monocytes, memory T cells, and dendritic cells to sites of tissue injury and infection. Expression of this proinflammatory chemokine is increased in atherosclerotic lesions, and inhibition of its expression or that of its receptor, CC chemokine receptor 2 (CCR2), reduces the extent of atheroma formation in hypercholesterolemic mice.
NCBI and Uniprot Product Information
NCBI Description
This gene is one of several cytokine genes clustered on chromosome 11. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and memory T cells but not for neutrophils. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis. [provided by RefSeq, Sep 2015]
Uniprot Description
CCL13: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens. By IL1/interleukin-1 and TNF. Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Secreted; Secreted, signal peptide; Chemokine; Motility/polarity/chemotaxis
Cellular Component: extracellular space; rough endoplasmic reticulum; cell soma; endocytic vesicle; perinuclear region of cytoplasm; dendrite; cytoplasm; extracellular region; synapse; perikaryon; nerve terminal
Molecular Function: heparin binding; protein binding; G-protein-coupled receptor binding; chemokine activity; CCR2 chemokine receptor binding; cytokine activity
Biological Process: positive regulation of cell adhesion; positive regulation of leukocyte migration; positive regulation of collagen biosynthetic process; chemotaxis; positive regulation of synaptic transmission; positive regulation of cellular extravasation; regulation of cell shape; transforming growth factor beta receptor signaling pathway; positive regulation of cell-cell adhesion; response to wounding; angiogenesis; inflammatory response; lymphocyte chemotaxis; neutrophil chemotaxis; cytokine and chemokine mediated signaling pathway; cytoskeleton organization and biogenesis; positive regulation of tumor necrosis factor production; glial cell migration; macrophage chemotaxis; leukocyte migration during inflammatory response; cellular calcium ion homeostasis; negative regulation of angiogenesis; positive regulation of leukocyte mediated cytotoxicity; response to heat; eosinophil chemotaxis; immune response; positive regulation of endothelial cell proliferation; positive regulation of T cell activation; vascular endothelial growth factor receptor signaling pathway; positive regulation of nitric-oxide synthase biosynthetic process
Research Articles on CCL2
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Product Notes
The Mouse CCL2 ccl2 (Catalog #AAA9711574) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711574 ELISA Kit recognizes Mouse CCL2. It is sometimes possible for the material contained within the vial of "CCL2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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