Rabbit anti-Human ERV3 Polyclonal Antibody | anti-ERV3 antibody

ERV3, CT (ERV3-1, ERV3, HERV-R_7q21.2 provirus ancestral Env polyprotein, ERV-3 envelope protein, ERV3-1 envelope protein, Envelope polyprotein, HERV-R envelope protein, Surface protein, Transmembrane protein) (PE)

Cat. Num. AAA6296689

• Gene Names: ERV3-1; ERV3; ERVR; envR; ERV-R; HERVR; HERV-R
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ERV3; Polyclonal Antibody; CT (ERV3-1; HERV-R_7q21.2 provirus ancestral Env polyprotein; ERV-3 envelope protein; ERV3-1 envelope protein; Envelope polyprotein; HERV-R envelope protein; Surface protein; Transmembrane protein) (PE); ERV3-1; Transmembrane protein; anti-ERV3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ERV3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ERV3 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 445-474 in the C-terminal region of human ERV3, UniProt Accession #Q14264; NP_001007254.2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in 293, HepG2 cell line lysates (35ug/lane) usingMBS6007937. This demonstrates that MBS6007937 detected the ERV3 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue using MBS6007937 followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MBS6007937 for immunohistochemistry.)

Related Product Information for anti-ERV3 antibody

The human genome includes many retroelements including the human endogenous retroviruses (HERVs). ERV3, one of the most studied HERVs, is thought to have integrated 30 to 40 million years ago and is present in higher primates with the exception of gorillas. Taken together, the observation of genome conservation, the detection of transcript expression, and the presence of conserved ORFs is circumstantial evidence for a functional role. A functional role is also suggested by the observation that downregulation of ERV3 is reported in choriocarcinoma. [provided by RefSeq].

Product Categories/Family for anti-ERV3 antibody

Antibodies; Infectious Disease; HERV

References

1. Andersson, A.C., et al. J. Virol. 79(14):9270-9284(2005). 2. Herve, C.A., et al. Genomics 83(5):940-943(2004). 3. Blaise, S., et al. Proc. Natl. Acad. Sci. U.S.A. 100(22):13013-13018(2003). 4. de Parseval, N., et al. J. Virol. 77(19):10414-10422(2003). 5. Andersson, A.C., et al. Virology 297(2):220-225(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 145651814
• NCBI GeneID: 2086
• NCBI Accession #: NP_001007254.2
• NCBI GenBank Nucleotide #: NM_001007253.3
• UniProt Accession #: Q14264
• NCBI Official Full Name: endogenous retrovirus group 3 member 1 Env polyprotein
• NCBI Official Synonym Full Names: endogenous retrovirus group 3 member 1
• NCBI Official Symbol: ERV3-1
• NCBI Official Synonym Symbols: ERV3; ERVR; envR; ERV-R; HERVR; HERV-R
• NCBI Protein Information: endogenous retrovirus group 3 member 1 Env polyprotein
• UniProt Protein Name: Endogenous retrovirus group 3 member 1 Env polyprotein
• UniProt Gene Name: ERV3-1
• UniProt Synonym Gene Names: ERV3; ERV3 envelope protein; ERV-R envelope protein; SU; TM
• UniProt Entry Name: ENR1_HUMAN

NCBI Description

The human genome includes many retroelements including the human endogenous retroviruses (HERVs). ERV3, one of the most studied HERVs, is thought to have integrated 30 to 40 million years ago and is present in higher primates with the exception of gorillas. Taken together, the observation of genome conservation, the detection of transcript expression, and the presence of conserved ORFs is circumstantial evidence for a functional role. A functional role is also suggested by the observation that downregulation of ERV3 is reported in choriocarcinoma. [provided by RefSeq, Jul 2008]

Uniprot Description

ERV3: Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. This endogenous envelope protein has lost its fusogenic properties. It can inhibit cell growth through decrease expression of cyclin B1 and increased expression of p21 in vitro. Belongs to the gamma type-C retroviral envelope protein family. HERV class-I R env subfamily.

Chromosomal Location of Human Ortholog: 7q11.2

Cellular Component: viral envelope