Rabbit ERCC1 Polyclonal Antibody | anti-ERCC1 antibody

ERCC1 Polyclonal Antibody

Cat. Num. AAA3011755

• Gene Names: ERCC1; UV20; COFS4; RAD10
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ERCC1; Polyclonal Antibody; ERCC1 Polyclonal Antibody; DNA excision repair protein ERCC-1; : ERCC1; anti-ERCC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ERCC1 polyclonal antibody detects endogenous levels of ERCC1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 273

• Applicable Applications for anti-ERCC1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: Recombinant full length Human ERCC1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using ERCC1 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells using ERCC1 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cells using ERCC1 antibody. Blue: DAPI for nuclear staining.)

Testing Data

Related Product Information for anti-ERCC1 antibody

Xeroderma pigmentosum (XP) is an autosomal recessive disorder characterized by a genetic predisposition to sunlight-induced skin cancer; it is commonly due to deficiencies in DNA repair enzymes. The most frequent mutations are found in the XP genes from group A through G and group V, which encode for nucleotide excision repair proteins. XPF, which is also designated ERCC4 or ERCC11, associates directly with the excision repair cross-complementing 1 (ERCC1) factor. ERCC1, a functional homolog of Rad10 in S. cerevisiae, is a component of a structure-specific endonuclease that is responsible for 5' incisions during DNA repair. The ERCC1-XPF endonuclease preferentially cleaves one strand of DNA between duplex and single-stranded regions near borders of the stem-loop structure and, thereby, contributes to the initial steps of the nucleotide excision repair process.

NCBI and Uniprot Product Information

• NCBI GI #: 260593725
• NCBI GeneID: 2067
• NCBI Accession #: NP_001159521.1
• NCBI GenBank Nucleotide #: NP_001159521.1
• UniProt Accession #: P07992
• Molecular Weight: ~ 38kDa
• NCBI Official Full Name: DNA excision repair protein ERCC-1 isoform 3
• NCBI Official Synonym Full Names: ERCC excision repair 1, endonuclease non-catalytic subunit
• NCBI Official Symbol: ERCC1
• NCBI Official Synonym Symbols: UV20; COFS4; RAD10
• NCBI Protein Information: DNA excision repair protein ERCC-1
• UniProt Protein Name: DNA excision repair protein ERCC-1
• Protein Family: DNA excision repair protein
• UniProt Gene Name: ERCC1
• UniProt Entry Name: ERCC1_HUMAN

NCBI Description

The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq, Oct 2009]

Uniprot Description

Function: Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.

Subunit structure: Heterodimer composed of ERCC1 and XPF/ERRC4.

Subcellular location: Nucleus.

Involvement in disease: Cerebro-oculo-facio-skeletal syndrome 4 (COFS4) [MIM:610758]: A disorder of prenatal onset characterized by microcephaly, congenital cataracts, facial dysmorphism, neurogenic arthrogryposis, growth failure and severe psychomotor retardation. COFS is considered to be part of the nucleotide-excision repair disorders spectrum that include also xeroderma pigmentosum, trichothiodystrophy and Cockayne syndrome.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.10

Sequence similarities: Belongs to the ERCC1/RAD10/SWI10 family.