Western Blot (WB)
(EMX1 Antibody (C-term) western blot analysis in U251 and Neuro-2a cell line mouse cerebellum tissue lysates (35ug/lane).This demonstrates the EMX1 antibody detected the EMX1 protein (arrow).)
Rabbit anti-Human, mouse EMX1 Polyclonal Antibody | anti-EMX1 antibody
EMX1 Antibody (C-term)
Western Blot (WB)
(EMX1 Antibody (C-term) western blot analysis in U251 and Neuro-2a cell line mouse cerebellum tissue lysates (35ug/lane).This demonstrates the EMX1 antibody detected the EMX1 protein (arrow).)
Western Blot (WB)
(EMX1 Antibody (C-term) western blot analysis in U251 and Neuro-2a cell line mouse cerebellum tissue lysates (35ug/lane).This demonstrates the EMX1 antibody detected the EMX1 protein (arrow).)
Flow Cytometry (FC/FACS)
(EMX1 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(EMX1 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with EMX1 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EMX1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). EMX1 immunoreactivity is localized to Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with EMX1 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EMX1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). EMX1 immunoreactivity is localized to Nucleus significantly.)
NCBI and Uniprot Product Information
Uniprot Description
EMX1: Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system. Belongs to the EMX homeobox family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 2p13.2
Cellular Component: cytoplasm; nucleus
Molecular Function: protein binding; sequence-specific DNA binding
Biological Process: response to drug; regulation of transcription, DNA-dependent; in utero embryonic development; brain morphogenesis; cerebral cortex regionalization; post-embryonic development; cerebral cortex neuron differentiation
Research Articles on EMX1
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Product Notes
The EMX1 emx1 (Catalog #AAA9200852) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 228-257. The EMX1 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EMX1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the EMX1 emx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EMX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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