Rabbit anti-Human, Mouse EMX1 Polyclonal Antibody | anti-EMX1 antibody

EMX1, CT (Empty Spiracles-like Protein 1, Empty Spiracles Homolog 1, Homeobox Protein EMX1) (Azide free) (HRP)

Cat. Num. AAA6473798

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: EMX1; Polyclonal Antibody; CT (Empty Spiracles-like Protein 1; Empty Spiracles Homolog 1; Homeobox Protein EMX1) (Azide free) (HRP); anti-EMX1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EMX1. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-EMX1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 228-257 in the C-terminal region of human EMX1, UniProt Accession #NP_004088.2; Q04741; Q04742.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in U251 and Neuro-2a cell line mouse cerebellum tissue lysates (35ug/lane) usingMBS624908. This demonstrates that MBS624908 detected the EMX1 protein (arrow).)

Testing Data

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Related Product Information for anti-EMX1 antibody

EMX1 is a transcription factor, which in cooperation with EMX2. It acts to generate the boundary between the roof and archipallium in the developing brain. It may function in combinations with OTX1/2 to specify cell fates in the developing central nervous system.

Product Categories/Family for anti-EMX1 antibody

Antibodies; Transcription Factors; Homeobox (HOX)

References

1. Briata, P., et.al., Mech. Dev. 57(2), 169-180 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 94536800
• NCBI GeneID: 2016
• NCBI Accession #: NP_004088.2
• NCBI GenBank Nucleotide #: NM_004097.2
• UniProt Accession #: Q04741; Q04742
• Molecular Weight: 28,039 Da
• NCBI Official Full Name: homeobox protein EMX1
• NCBI Official Synonym Full Names: empty spiracles homeobox 1
• NCBI Official Symbol: EMX1
• NCBI Protein Information: homeobox protein EMX1; empty spiracles homolog 1; empty spiracles-like protein 1
• UniProt Protein Name: Homeobox protein EMX1
• Protein Family: Homeobox protein
• UniProt Gene Name: EMX1
• UniProt Entry Name: EMX1_HUMAN

Uniprot Description

EMX1: Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system. Belongs to the EMX homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 2p13.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; sequence-specific DNA binding

Biological Process: response to drug; regulation of transcription, DNA-dependent; in utero embryonic development; brain morphogenesis; cerebral cortex regionalization; cerebral cortex neuron differentiation; post-embryonic development