Mouse Beta-2-glycoprotein 1 ELISA Kit | Apoh elisa kit

Mouse Beta-2-glycoprotein 1 ELISA Kit

Cat. Num. AAA761877

• Gene Names: APOH; BG; B2G1; B2GP1
• Reactivity: Mouse
• Synonyms: Beta-2-glycoprotein 1; Mouse Beta-2-glycoprotein 1 ELISA Kit; Apoh/APOH/B2G1/B2GPI/Beta/BG/Activated protein C-binding protein/Anticardiolipin cofactor/APC inhibitor/Apo-H/apo-H/Apolipoprotein H/apolipoprotein H (beta-2-glycoprotein I)/B2G1/B2GP1/B2GPI/Beta (2)GPI/beta (2)GPI/beta-2-glycoprotein 1/Beta-2-glycoprotein I/BG; Apoh elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Apoh. No significant cross-reactivity or interference between Apoh and analogues was observed.
• Sequence Length: 345

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 12.5-800ng/ml
• Sensitivity: 7.5ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Apoh elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 153266841
• NCBI GeneID: 350
• NCBI Accession #: NP_000033.2
• NCBI GenBank Nucleotide #: NM_000042.2
• Molecular Weight: 38,298 Da
• NCBI Official Full Name: beta-2-glycoprotein 1
• NCBI Official Synonym Full Names: apolipoprotein H
• NCBI Official Symbol: APOH
• NCBI Official Synonym Symbols: BG; B2G1; B2GP1
• NCBI Protein Information: beta-2-glycoprotein 1
• UniProt Protein Name: Beta-2-glycoprotein 1
• Protein Family: Beta-2-glycoprotein
• UniProt Gene Name: APOH
• UniProt Synonym Gene Names: B2G1; Apo-H; B2GPI; Beta(2)GPI
• UniProt Entry Name: APOH_HUMAN

NCBI Description

Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections. [provided by RefSeq, Jul 2008]

Uniprot Description

APOH: Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.

Protein type: Lipid-binding; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: cell surface; chylomicron; extracellular matrix; extracellular region; extracellular space

Molecular Function: glycoprotein binding; identical protein binding; lipid binding; phospholipid binding; protein binding

Biological Process: blood coagulation, intrinsic pathway; negative regulation of angiogenesis; negative regulation of blood coagulation; negative regulation of endothelial cell proliferation; negative regulation of fibrinolysis; negative regulation of myeloid cell apoptosis; plasminogen activation; platelet degranulation; positive regulation of blood coagulation; positive regulation of lipoprotein lipase activity; regulation of fibrinolysis; triacylglycerol metabolic process; triacylglycerol transport