Rabbit anti-Human CHRNA7 Polyclonal Antibody | anti-CHRNA7 antibody

CHRNA7, NT (CHRNA7, NACHRA7, Neuronal acetylcholine receptor subunit alpha-7) (AP)

Cat. Num. AAA6285865

• Gene Names: CHRNA7; NACHRA7; CHRNA7-2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CHRNA7; Polyclonal Antibody; NT (CHRNA7; NACHRA7; Neuronal acetylcholine receptor subunit alpha-7) (AP); Neuronal acetylcholine receptor subunit alpha-7; anti-CHRNA7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CHRNA7 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CHRNA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-37 amino acids from the N-terminal region of human CHRNA7.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CHRNA7 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CHRNA7 antibody detected the CHRNA7 protein (arrow).)

Related Product Information for anti-CHRNA7 antibody

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-CHRNA7 antibody

Antibodies; Receptors

References

Chernyavsky, A.I., et al. Am. J. Physiol., Cell Physiol. 299 (5), C903-C911 (2010): Saccone, N.L., et al. Genes Brain Behav. 9(7):741-750(2010). Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010). Jin, Y., et al. Int. J. Immunogenet. (2010). In press: Schraufstatter, I.U., et al. J Stem Cells 4(4):203-215(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4502831
• NCBI GeneID: 1139
• NCBI Accession #: NP_000737.1
• NCBI GenBank Nucleotide #: NM_000746.5
• UniProt Accession #: P36544; Q15826; Q8IUZ4; Q96RH2; Q99555; Q9BXH0; A8K7Q4; B4DFS0
• Molecular Weight: 35,482 Da
• NCBI Official Full Name: neuronal acetylcholine receptor subunit alpha-7 isoform 1
• NCBI Official Synonym Full Names: cholinergic receptor, nicotinic, alpha 7 (neuronal)
• NCBI Official Symbol: CHRNA7
• NCBI Official Synonym Symbols: NACHRA7; CHRNA7-2
• NCBI Protein Information: neuronal acetylcholine receptor subunit alpha-7; a7 nicotinic acetylcholine receptor; alpha-7 nicotinic cholinergic receptor subunit; alpha 7 neuronal nicotinic acetylcholine receptor; cholinergic receptor, nicotinic, alpha polypeptide 7; neuronal acetylc
• UniProt Protein Name: Neuronal acetylcholine receptor subunit alpha-7
• Protein Family: CHRNA7-FAM7A fusion protein
• UniProt Gene Name: CHRNA7
• UniProt Synonym Gene Names: NACHRA7
• UniProt Entry Name: ACHA7_HUMAN

NCBI Description

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Uniprot Description

nAChRA7: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha- 7/CHRNA7 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, cation; Membrane protein, multi-pass; Channel, ligand-gated; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: nicotinic acetylcholine-gated receptor-channel complex; postsynaptic membrane; plasma membrane; integral to membrane; cell junction

Molecular Function: toxin binding; chloride channel regulator activity; protein homodimerization activity; beta-amyloid binding; acetylcholine-gated cation channel activity; acetylcholine receptor activity; nicotinic acetylcholine-activated cation-selective channel activity; acetylcholine binding

Biological Process: response to nicotine; cellular calcium ion homeostasis; synaptic transmission; positive regulation of angiogenesis; activation of MAPK activity; calcium ion transport; positive regulation of cell proliferation; response to hypoxia; ion transport; negative regulation of tumor necrosis factor production; signal transduction; cognition; memory

Disease: Chromosome 15q13.3 Deletion Syndrome