Western Blot (WB)
(Western BlotPositive WB detected in: K562 whole cell lysate,HL-60 whole cell lysate,Hela whole cell lysate,MCF-7 whole cell lysate,HepG2 whole cell lysate,Rat liver tissue,Mouse brain tissue,Mouse lung tissueAll lanes: EIF3D antibody at 2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 64,59,63 KDaObserved band size: 64 KDa)
Rabbit anti-Human EIF3D Polyclonal Antibody | anti-EIF3D antibody
EIF3D Antibody
Western Blot (WB)
(Western BlotPositive WB detected in: K562 whole cell lysate,HL-60 whole cell lysate,Hela whole cell lysate,MCF-7 whole cell lysate,HepG2 whole cell lysate,Rat liver tissue,Mouse brain tissue,Mouse lung tissueAll lanes: EIF3D antibody at 2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 64,59,63 KDaObserved band size: 64 KDa)
Western Blot (WB)
(Western BlotPositive WB detected in: K562 whole cell lysate,HL-60 whole cell lysate,Hela whole cell lysate,MCF-7 whole cell lysate,HepG2 whole cell lysate,Rat liver tissue,Mouse brain tissue,Mouse lung tissueAll lanes: EIF3D antibody at 2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 64,59,63 KDaObserved band size: 64 KDa)
Immunohistochemistry (IHC)
(IHC image of MBS7006714 diluted at 1:200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC)
(IHC image of MBS7006714 diluted at 1:200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC)
(IHC image of MBS7006714 diluted at 1:200 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC)
(IHC image of MBS7006714 diluted at 1:200 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
NCBI and Uniprot Product Information
NCBI Description
Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex. [provided by RefSeq, Jul 2008]
Uniprot Description
eIF3-zeta: eukaryotic translation initiation factor 3 subunit 7. Binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. Associates with the subunit p170 of eIF-3. eIF-3 is composed of at least 12 different subunits.
Protein type: RNA-binding; Translation initiation; Translation
Chromosomal Location of Human Ortholog: 22q13.1
Cellular Component: cytosol; eukaryotic translation initiation factor 3 complex; membrane
Molecular Function: protein binding; translation initiation factor activity
Biological Process: cellular protein metabolic process; formation of translation initiation complex; formation of translation preinitiation complex; gene expression; regulation of translational initiation; translation; translational initiation
Research Articles on EIF3D
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Product Notes
The EIF3D eif3d (Catalog #AAA7006714) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EIF3D Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EIF3D can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the EIF3D eif3d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF3D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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