ubc7 (ubiquitin Conjugating enzyme 7) Recombinant Protein | ubc7 recombinant protein

ubc7 (ubiquitin Conjugating enzyme 7), 1-154 aa, Human, E Coli

Cat. Num. AAA203071

• Gene Names: UBE2L3; E2-F1; L-UBC; UBCH7; UbcM4
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: ubc7 (ubiquitin Conjugating enzyme 7); 1-154 aa; Human; E Coli; ubiquitin-conjugating enzyme E2L 3; uBE2G1; uBE2G; EC 6.3.2.19; ubiquitin-protein ligase G1; ubiquitin carrier protein G1; E217K; uBC7; ubiquitin-conjugating enzyme E2 G1; ubiquitin-conjugating enzyme E2L 3 uBC 7; uBC7 homolog yeast; uBE 2G; ubiquitin conjugating enzyme E2 G1; ubiquitin conjugating enzyme E2G 1 (homologous to C; elegans uBC7); ubiquitin conjugating enzyme E2G 1 (uBC7 homolog C; elegans) antibody; ubiquitin conjugating enzyme E2G 1 (uBC7 homolog yeast); ubiquitin conjugating enzyme E2G 1; ubiquitin protein ligase G1; ; ubc7 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 50 mM HEPES (pH 7.5) 150 mM NaCl, 1mM DTT, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MAASRRLMKE LEEIRKCGMK NFRNIQVDEA NLLTWQGLIV PDNPPYDKGA FRIEINFPAE YPFKPPKITF KTKIYHPNID EKGQVCLPVI SAENWKPATK TDQVIQSLIA LVNDPQPEHP LRADLAEEYS KDRKKFCKNA EEFTKKYGEK RPVD
• Sequence Length: 154

• Applicable Applications for ubc7 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for ubc7 recombinant protein

Human ubquitin-conjugating enzyme 7 (ubcH7) is a ubiquitin-conjugating enzyme (E2) mediating c-fos degradation, transcription factor NF-kappa B maturation, and human papilloma virus-mediated p53 and Myc protein degradation, in vitro. The ubiquitin-conjugating enzymes (E2s) are essential components of the post-translational protein ubiquitination pathway, mediating the transfer of activated ubiquitin to substrate proteins. The human uBE2L1-uBE2L4 gene could potentially encode different isoforms of the ubcH7. uBE2L3 gene, located at chromosome 22q11.2, is the only identical family member with introns and encodes a polypeptide sequence identical to that of ubcH7. ubcH7 (154amino acid) was over-expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for ubc7 recombinant protein

Ubiquitination

References

Ardley HC., et al. (2000) Biochim Biophys Acta. 1491 (1-3):57-64.; Moynihan TP., et al. (1998) Genomics. 51 (1):124-127.; Nuber u., et al (1996) J Biol Chem. 271 (5):2795-2800;

NCBI and Uniprot Product Information

• NCBI GI #: 4507789
• NCBI GeneID: 7332
• NCBI Accession #: NP_003338
• NCBI GenBank Nucleotide #: NM_003347.3
• UniProt Accession #: P68036; P51966; P70653; Q9HAV1; B2R4A7; B4DDG1; B4DSZ4; E7EWS7
• Molecular Weight: 17.9 kDa (154 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 L3 isoform 1
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 L3
• NCBI Official Symbol: UBE2L3
• NCBI Official Synonym Symbols: E2-F1; L-UBC; UBCH7; UbcM4
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 L3
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 L3
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2L3
• UniProt Synonym Gene Names: UBCE7; UBCH7

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PRKN and ARIH1, that function like function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.