Rabbit anti-Human, Mouse EIF2AK2 Polyclonal Antibody | anti-EIF2AK2 antibody

EIF2AK2, NT (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2, eIF-2A Protein Kinase 2, Interferon-induced, Double-stranded RNA-activated Protein Kinase, Interferon-inducible RNA-dependent pRotein Kinase, p68 kinase, Protein Kinase RNA-activated,

Cat. Num. AAA628941

• Gene Names: EIF2AK2; PKR; PRKR; EIF2AK1; MGC126524
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: EIF2AK2; Polyclonal Antibody; NT (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2; eIF-2A Protein Kinase 2; Interferon-induced; Double-stranded RNA-activated Protein Kinase; Interferon-inducible RNA-dependent pRotein Kinase; p68 kinase; Protein Kinase RNA-activated; ; Anti -EIF2AK2; anti-EIF2AK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRKR. Species Crossreactivity: mouse.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-EIF2AK2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the N-terminal region of human PRKR (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-PRKR Pab is used in Western blot to detect PRKR in mouse liver tissue lysate (Lane 1) and HepG2 cell lysate (Lane 2).)

Western Blot (WB)

(Western blot analysis of EIF2AK2 (arrow) using PRKR Antibody (N-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF2AK2 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-EIF2AK2 antibody

Interferon-induced, double-stranded RNA-activated protein kinase (PRKR) is a serine-threonine kinase. Activation by dsRNAs leads to autophosphorylation of PRKR and allows the kinase to phosphorylate its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor-2 (EIF2-alpha), leading to the inhibition of protein synthesis. Human gamma-interferon (IFNG) mRNA exploits localized activation of PRKR in the cell to regulate its own translation. IFNG mRNA activates PRKR through a pseudoknot in its 5-prime untranslated region. The HCV envelope protein E2 contains a sequence identical with phosphorylation sites of the interferon-inducible protein kinase PRKR and the translation initiation factor EIF2-alpha, a target of PRKR. E2 inhibits the kinase activity of PRKR and blocks its inhibitory effect on protein synthesis and cell growth, which provides one mechanism by which HCV may circumvent the antiviral effect of interferon. PRKR, which is involved in TLR signaling and mediates apoptosis in fibroblasts in response to viral infection and inflammatory cytokines, also activates IKK and NFKB, thereby suppressing apoptosis. Apoptosis induced by live pathogenic gram-positive and gram-negative bacteria requirs both TLR4 and PRKR, possibly representing a major mechanism for pathogenic bacteria that use specific virulence factors to avoid detection and destruction by the innate immune system. Roles for PRKR activation in Huntington disease and Fanconi anemia have also been suggested.

Product Categories/Family for anti-EIF2AK2 antibody

Antibodies; Abs to Translation Initiation Factors

NCBI and Uniprot Product Information

• NCBI GI #: 33871554
• NCBI GeneID: 5610
• UniProt Accession #: Q6PK38; P19525; Q05CP4; Q7Z6F6; Q8IW76
• Molecular Weight: 42,929 Da
• NCBI Official Full Name: EIF2AK2 protein
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2-alpha kinase 2
• NCBI Official Symbol: EIF2AK2
• NCBI Official Synonym Symbols: PKR; PRKR; EIF2AK1; MGC126524
• NCBI Protein Information: interferon-induced, double-stranded RNA-activated protein kinase; p68 kinase; OTTHUMP00000126954; OTTHUMP00000201320; eIF-2A protein kinase 2; P1/eIF-2A protein kinase; protein kinase RNA-activated; interferon-inducible elF2alpha kinase; double stranded RNA activated protein kinase; interferon-inducible RNA-dependent protein kinase; protein kinase, interferon-inducible double stranded RNA dependent
• UniProt Protein Name: EIF2AK2 protein
• Protein Family: Interferon-induced, double-stranded RNA-activated protein kinase
• UniProt Gene Name: EIF2AK2
• UniProt Entry Name: Q6PK38_HUMAN

NCBI Description

EIF2AK2 is a protein serine/threonine kinase that acquires enzymatic activity following autophosphorylation, a process mediated by double-stranded RNA (dsRNA). Activation of EIF2AK2 allows the kinase to phosphorylate its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor-2 (EIF2-alpha; MIM 603907), leading to the inhibition of protein synthesis (summary by Kuhen et al., 1996 [PubMed 8812437]).[supplied by OMIM]

Uniprot Description

PKR: a protein kinase of the PEK family. Upon binding double-stranded RNA, it becomes autophosphorylated and activated. Phosphorylates and inhibits the alpha subunit of eIF2 alpha, which leads to an inhibition of the initiation of protein synthesis. Controls the activation of several transcription factors such as NF-kappaB, p53 and Stats. Mediates apoptosis induced by many different stimuli, such as LPS, TNF-alpha, viral infection and serum starvation.

Protein type: Kinase, protein; Protein kinase, Other; EC 2.7.10.2; Translation; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Other group; PEK family

Chromosomal Location of Human Ortholog: 2p22-p21

Cellular Component: membrane; perinuclear region of cytoplasm; cytoplasm; ribosome; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; double-stranded RNA binding; non-membrane spanning protein tyrosine kinase activity; protein phosphatase type 2A regulator activity; eukaryotic translation initiation factor 2alpha kinase activity; ATP binding; protein kinase activity

Biological Process: positive regulation of cytokine production; peptidyl-tyrosine phosphorylation; translation; activation of MAPKK activity; transcription, DNA-dependent; unfolded protein response; protein amino acid autophosphorylation; response to virus; viral infectious cycle; protein amino acid phosphorylation; evasion by virus of host immune response; positive regulation of chemokine production; positive regulation of stress-activated MAPK cascade; activation of NF-kappaB transcription factor; negative regulation of cell proliferation; negative regulation of viral genome replication; modification by virus of host cellular process; negative regulation of translation; virus-host interaction; innate immune response; negative regulation of osteoblast proliferation; defense response to virus; negative regulation of apoptosis