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Western Blot (WB) (Western Blot detection against Immunogen (37.84kD).)

Mouse anti-Human ARRB2 Monoclonal Antibody | anti-ARRB2 antibody

ARRB2 (Beta-arrestin-2, Arrestin beta-2, ARB2, ARR2) APC

Gene Names
ARRB2; ARB2; ARR2; BARR2
Reactivity
Human
Applications
ELISA, Immunofluorescence, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ARRB2; Monoclonal Antibody; ARRB2 (Beta-arrestin-2; Arrestin beta-2; ARB2; ARR2) APC; anti-ARRB2 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3G1
Specificity
Recognizes human ARRB2.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-ARRB2 antibody
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Application Notes
IF: 10ug/ml
Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa300-409 from human ARRB2 (AAH07427) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
NLASSTIVKEGANKEVLGILVSYRVKVKLVVSRGGDVSVELPFVLMHPKPHDHIPLPRPQSAAPETDVPVDTNLIEFDTNYATDDDIVFEDFARLRLKGMKDDDYDDQLC
Conjugate
APC
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (37.84kD).)

Western Blot (WB) (Western Blot detection against Immunogen (37.84kD).)

Western Blot (WB)

(Western Blot analysis of ARRB2 expression in transfected 293T cell line by ARRB2 monoclonal antibody. Lane 1: ARRB2 transfected lysate (46kD). Lane 2: Non-transfected lysate.)

Western Blot (WB) (Western Blot analysis of ARRB2 expression in transfected 293T cell line by ARRB2 monoclonal antibody. Lane 1: ARRB2 transfected lysate (46kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to ARRB2 on HeLa cell. [antibody concentration 10ug/ml].)

Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to ARRB2 on HeLa cell. [antibody concentration 10ug/ml].)
Product Categories/Family for anti-ARRB2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
409
Molecular Weight
44,392 Da
NCBI Official Full Name
Homo sapiens arrestin, beta 2, mRNA
NCBI Official Synonym Full Names
arrestin beta 2
NCBI Official Symbol
ARRB2
NCBI Official Synonym Symbols
ARB2; ARR2; BARR2
NCBI Protein Information
beta-arrestin-2
Protein Family

NCBI Description

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]

Research Articles on ARRB2

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Product Notes

The ARRB2 (Catalog #AAA6135380) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ARRB2 (Beta-arrestin-2, Arrestin beta-2, ARB2, ARR2) APC reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARRB2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Western Blot (WB). IF: 10ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ARRB2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARRB2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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