Western Blot (WB)
(Anti-PKR antibody, MBS176094, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant Human EIF2AK2, 35.0KD(162aa tag+ M1-K154)Lane 1: Recombinant Human EIF2AK2 Protein 10ngLane 2: Recombinant Human EIF2AK2 Protein 5ngLane 3: Recombinant Human EIF2AK2 Protein 2.5ngLane 4: Recombinant Human EIF2AK2 Protein 1.25ng )
Rabbit anti-Human PKR Polyclonal Antibody | anti-EIF2AK2 antibody
Anti-PKR antibody
Western Blot (WB)
(Anti-PKR antibody, MBS176094, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant Human EIF2AK2, 35.0KD(162aa tag+ M1-K154)Lane 1: Recombinant Human EIF2AK2 Protein 10ngLane 2: Recombinant Human EIF2AK2 Protein 5ngLane 3: Recombinant Human EIF2AK2 Protein 2.5ngLane 4: Recombinant Human EIF2AK2 Protein 1.25ng )
Western Blot (WB)
(Anti-PKR antibody, MBS176094, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant Human EIF2AK2, 35.0KD(162aa tag+ M1-K154)Lane 1: Recombinant Human EIF2AK2 Protein 10ngLane 2: Recombinant Human EIF2AK2 Protein 5ngLane 3: Recombinant Human EIF2AK2 Protein 2.5ngLane 4: Recombinant Human EIF2AK2 Protein 1.25ng )
Background: EIF2AK2(Eukaryotic Translation Initiation Factor 2-Alpha Kinase 2), also called PKR, is an enzyme that in humans is encoded by the EIF2AK2 gene. Activation of EIF2AK2 allows the kinase to phosphorylate its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor-2, leading to the inhibition of protein synthesis(summary by Kuhen et al., 1996). By FISH analysis, Squire et al.(1993) assigned the EIF2AK2 gene to the boundary between chromosome 2p22-p21. Ben-Asouli et al.(2002) showed that human gamma-interferon mRNA uses local activation of PKR in the cell to control its own translation yield. IFNG mRNA was found to activate PKR through a pseudoknot in its 5-prime untranslated region. Taylor et al.(1999) studied the mechanism underlying the resistance of hepatitis C virus(HCV) to interferon. They demonstrated that the HCV envelope protein E2 contains a sequence identical with phosphorylation sites of the interferon-inducible protein kinase PKR and the translation initiation factor EIF2-alpha, a target of PKR. E2 inhibited the kinase activity of PKR and blocked its inhibitory effect on protein synthesis and cell growth.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
PKR: a protein kinase of the PEK family. Upon binding double-stranded RNA, it becomes autophosphorylated and activated. Phosphorylates and inhibits the alpha subunit of eIF2 alpha, which leads to an inhibition of the initiation of protein synthesis. Controls the activation of several transcription factors such as NF-kappaB, p53 and Stats. Mediates apoptosis induced by many different stimuli, such as LPS, TNF-alpha, viral infection and serum starvation.
Protein type: Protein kinase, Other; EC 2.7.10.2; EC 2.7.11.1; Translation; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Other group; PEK family
Chromosomal Location of Human Ortholog: 2p22-p21
Cellular Component: membrane; perinuclear region of cytoplasm; cytoplasm; ribosome; cytosol; nucleus
Molecular Function: protein serine/threonine kinase activity; protein binding; double-stranded RNA binding; non-membrane spanning protein tyrosine kinase activity; protein phosphatase type 2A regulator activity; ATP binding; eukaryotic translation initiation factor 2alpha kinase activity; protein kinase activity
Biological Process: positive regulation of cytokine production; peptidyl-tyrosine phosphorylation; transcription, DNA-dependent; translation; activation of MAPKK activity; unfolded protein response; protein amino acid autophosphorylation; response to virus; viral infectious cycle; protein amino acid phosphorylation; evasion by virus of host immune response; positive regulation of stress-activated MAPK cascade; positive regulation of chemokine production; activation of NF-kappaB transcription factor; negative regulation of cell proliferation; modification by virus of host cellular process; negative regulation of viral genome replication; virus-host interaction; negative regulation of translation; innate immune response; negative regulation of osteoblast proliferation; defense response to virus; negative regulation of apoptosis
Research Articles on EIF2AK2
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Product Notes
The EIF2AK2 eif2ak2 (Catalog #AAA176094) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PKR antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PKR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the EIF2AK2 eif2ak2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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