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Immunohistochemistry (IHC) (Anti-EDF1/MBF1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Goat EDF1/MBF1 Polyclonal Antibody | anti-EDF1/MBF1 antibody

Anti-EDF1/MBF1 Antibody (aa49-63)

Gene Names
EDF1; MBF1; EDF-1; CFAP280
Reactivity
Human
Predicted: Mouse, Guinea pig, Bovine, Rat, Pig, Horse, Monkey
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Immunoaffinity Purified
Synonyms
EDF1/MBF1; Polyclonal Antibody; Anti-EDF1/MBF1 Antibody (aa49-63); Rabbit Polyclonal (IgG) to Human EDF1/MBF1; EDF1; EDF-1; Multiprotein bridging factor 1; Multiprotein-bridging factor 1; MBF1; anti-EDF1/MBF1 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Human
Predicted: Mouse, Guinea pig, Bovine, Rat, Pig, Horse, Monkey
Clonality
Polyclonal
Specificity
Human EDF1/MBF1. This antibody is expected to recognize both reported isoforms (NP_003783.1; NP_694880.1).
Purity/Purification
Immunoaffinity Purified
Form/Format
Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Sequence Length
148
Applicable Applications for anti-EDF1/MBF1 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
ELISA: 1:128000
IHC-P: 5 ug/ml
WB: 0.3-1 ug/ml
Immunogen
Synthetic peptide C-NKQHSITKNTAKLDR from an internal region of human EDF1/MBF1 (NP_003783.1; NP_694880.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Mouse, Rat, Bovine, Elephant, Panda, Horse, Pig, Guinea pig, Platypus, Lizard (100%); Ferret, Turkey, Zebra finch, Chicken, Xenopus, Trout, Pike, Catfish, Salmon, Smelt (93%); Zebrafish, Drosophila (87%).
Conjugation
Unconjugated
Epitope
aa49-63
Preparation and Storage
Store at -20 degree C.
Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-EDF1/MBF1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC) (Anti-EDF1/MBF1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(EDF1 antibody (0.3 ug/ml) staining of A549 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) (EDF1 antibody (0.3 ug/ml) staining of A549 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Related Product Information for anti-EDF1/MBF1 antibody
Transcriptional coactivator stimulating NR5A1 and ligand-dependentNR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitricoxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,636 Da
NCBI Official Full Name
endothelial differentiation-related factor 1 isoform alpha
NCBI Official Synonym Full Names
endothelial differentiation related factor 1
NCBI Official Symbol
EDF1
NCBI Official Synonym Symbols
MBF1; EDF-1; CFAP280
NCBI Protein Information
endothelial differentiation-related factor 1
UniProt Protein Name
Endothelial differentiation-related factor 1
UniProt Gene Name
EDF1
UniProt Synonym Gene Names
EDF-1; MBF1

NCBI Description

This gene encodes a protein that may regulate endothelial cell differentiation, lipid metabolism, and hormone-induced cardiomyocyte hypertrophy. The encoded protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism.

Research Articles on EDF1/MBF1

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Product Notes

The EDF1/MBF1 edf1 (Catalog #AAA2400977) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Anti-EDF1/MBF1 Antibody (aa49-63) reacts with Human Predicted: Mouse, Guinea pig, Bovine, Rat, Pig, Horse, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's EDF1/MBF1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). ELISA: 1:128000 IHC-P: 5 ug/ml WB: 0.3-1 ug/ml. Researchers should empirically determine the suitability of the EDF1/MBF1 edf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EDF1/MBF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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