Western Blot (WB)
(Western blot analysis of Mouse brain lysate showing detection of ~67.5 kDa RYK protein using Rabbit Anti-RYK Polyclonal Antibody (SPC-740). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain lysate. Load: 15 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Rabbit Anti-RYK Polyclonal Antibody (SPC-740) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~67.5 kDa. Other Band(s): ~37 kDa degradation product.)
Rabbit anti-Mouse; Rat RYK Polyclonal Antibody | anti-RYK antibody
RYK Antibody: PerCP
Western Blot (WB)
(Western blot analysis of Mouse brain lysate showing detection of ~67.5 kDa RYK protein using Rabbit Anti-RYK Polyclonal Antibody (SPC-740). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain lysate. Load: 15 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Rabbit Anti-RYK Polyclonal Antibody (SPC-740) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~67.5 kDa. Other Band(s): ~37 kDa degradation product.)
Western Blot (WB)
(Western blot analysis of Mouse brain lysate showing detection of ~67.5 kDa RYK protein using Rabbit Anti-RYK Polyclonal Antibody (SPC-740). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain lysate. Load: 15 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Rabbit Anti-RYK Polyclonal Antibody (SPC-740) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~67.5 kDa. Other Band(s): ~37 kDa degradation product.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. The encoded protein has a leucine-rich extracellular domain with a WIF-type Wnt binding region, a single transmembrane domain, and an intracellular tyrosine kinase domain. This protein is involved in stimulating Wnt signaling pathways such as the regulation of axon pathfinding. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]
Uniprot Description
May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development.
Research Articles on RYK
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Product Notes
The RYK ryk (Catalog #AAA8000766) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RYK Antibody: PerCP reacts with Mouse; Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RYK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000. Researchers should empirically determine the suitability of the RYK ryk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RYK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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