Immunohistochemistry (IHC)
(Anti-ECSIT antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Rabbit anti-Human, Mouse ECSIT Polyclonal Antibody | anti-ECSIT antibody
Rabbit Polyclonal to Human ECSIT
Gene Names
ECSIT; SITPEC
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Ion exchange Chromatography
Synonyms
ECSIT; Polyclonal Antibody; Rabbit Polyclonal to Human ECSIT; Anti-ECSIT Antibody (C-Terminus) IHC-plus; ECSIT homolog (Drosophila); Protein SITPEC; SITPEC; Human ECSIT; anti-ECSIT antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
peptide corresponding to 14 amino acids near the C-terminus of human ECSIT
Purity/Purification
Ion exchange Chromatography
Form/Format
PBS, 0.02% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
431
Applicable Applications for anti-ECSIT antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes
IHC-P (5 ug/ml), WB (0.5 - 1 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
Target Species
Human
Immunogen Description
A peptide corresponding to 14 amino acids near the C-terminus of human ECSIT.
Immunogen Type
Synthetic peptide
Immunogen
ECSIT antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human ECSIT.
Antigen Modification
C-Terminus
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
Immunohistochemistry (IHC)
(Anti-ECSIT antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC)
(Anti-ECSIT antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Western Blot (WB)
(Western blot of ECSIT in human heart cell lysates with ECSIT antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.)
Western Blot (WB)
(Western blot of ECSIT in human heart cell lysates with ECSIT antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.)
Related Product Information for anti-ECSIT antibody
Activation of NF-kB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 (2) to kinases such as IRAK (3) and other intermediates such as TNF receptor associated factor (TRAF)-6.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,938 Da
NCBI Official Full Name
evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial isoform 1
NCBI Official Synonym Full Names
ECSIT signalling integrator
NCBI Official Symbol
ECSIT
NCBI Official Synonym Symbols
SITPEC
NCBI Protein Information
evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial; ECSIT homolog; likely ortholog of mouse signaling intermediate in Toll pathway evolutionarily conserved
UniProt Protein Name
Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial
Protein Family
UniProt Gene Name
ECSIT
UniProt Entry Name
ECSIT_HUMAN
Uniprot Description
ECSIT: Adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development. Required for efficient assembly of mitochondrial NADH:ubiquinone oxidoreductase. Belongs to the ECSIT family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: nucleoplasm; transcription factor complex; mitochondrion; cytoplasm; nucleus
Molecular Function: protein binding; signal transducer activity; oxidoreductase activity, acting on NADH or NADPH; transcription factor activity
Biological Process: BMP signaling pathway; mesoderm formation; innate immune response; regulation of oxidoreductase activity
Research Articles on ECSIT
Similar Products
Product Notes
The ECSIT ecsit (Catalog #AAA241267) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human ECSIT reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ECSIT can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB). IHC-P (5 ug/ml), WB (0.5 - 1 ug/ml) Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the ECSIT ecsit for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ECSIT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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