Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human colon tissue using MBS7611842(ECD antibody) at dilution of 1:100)
Rabbit anti-Human ECD Polyclonal Antibody | anti-ECD antibody
anti-ECD antibody
Gene Names
ECD; GCR2; HSGT1
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
>=95% as determined by SDS-PAGE. Immunogen affinity purified
Synonyms
ECD; Polyclonal Antibody; anti-ECD antibody; GCR2; HSGT1; Protein ecdysoneless homolog; Protein SGT1; SGT1; Suppressor of GCR2
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>=95% as determined by SDS-PAGE. Immunogen affinity purified
Form/Format
Liquid. PBS with 0.02% sodium azide and 50% glycerol pH7.3
Applicable Applications for anti-ECD antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:20-1:200
IHC: 1:20-1:200
Immunogen
ecdysoneless homolog(Drosophila)
Preparation and Storage
Store at -20 degree C for 12 months. Avoid repeated freeze/thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human colon tissue using MBS7611842(ECD antibody) at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human colon tissue using MBS7611842(ECD antibody) at dilution of 1:100)
Western Blot (WB)
(HeLa cells were subjected to SDS PAGE followed by western blot with MBS7611842(ECD antibody) at dilution of 1:1000)
Western Blot (WB)
(HeLa cells were subjected to SDS PAGE followed by western blot with MBS7611842(ECD antibody) at dilution of 1:1000)
Related Product Information for anti-ECD antibody
Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP(PubMed:16849563, PubMed:23880345). May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes(PubMed:19919181, PubMed:9928932). Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins(PubMed:19640839). The cell cycle-regulating function may depend on its RUVBL1-mediated association with the R2TP complex(PubMed:26711270). May play a role in regulation of pre-mRNA splicing(PubMed:24722212).
Product Categories/Family for anti-ECD antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
644
NCBI Official Full Name
Protein SGT1
NCBI Official Synonym Full Names
ecdysoneless homolog (Drosophila)
NCBI Official Symbol
ECD
NCBI Official Synonym Symbols
GCR2; HSGT1
NCBI Protein Information
protein SGT1; suppressor of GCR2; protein ecdysoneless homolog; suppressor of S. cerevisiae gcr2
UniProt Protein Name
Protein SGT1
Protein Family
UniProt Gene Name
ECD
UniProt Synonym Gene Names
SGT1; hSGT1
UniProt Entry Name
SGT1_HUMAN
Uniprot Description
SGT1: Novel regulator of p53 stability and function. May also be a transcriptional activator required for the expression of glycolytic genes. Belongs to the SGT1 family.
Protein type: Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 10q22.3
Cellular Component: nucleoplasm; cytoplasm
Molecular Function: protein binding; transcription coactivator activity
Biological Process: transcription from RNA polymerase II promoter; cell proliferation; regulation of transcription, DNA-dependent; regulation of glycolysis
Research Articles on ECD
Similar Products
Product Notes
The ECD ecd (Catalog #AAA7611842) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The anti-ECD antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ECD can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:20-1:200. Researchers should empirically determine the suitability of the ECD ecd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ECD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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