Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using ECD Antibody. The lane on the left was treated with blocking peptide.)
Rabbit ECD Polyclonal Antibody | anti-ECD antibody
ECD Antibody
Gene Names
ECD; GCR2; SGT1; HSGT1
Reactivity
Human, MousePredicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
ECD; Polyclonal Antibody; ECD Antibody; ecd; ECD_HUMAN; Ecdysoneless homolog; hSGT1; Human suppressor of GCR two; Protein ecdysoneless homolog; SGT 1; SGT1 protein; Suppressor of GCR2; anti-ECD antibody
Host
Rabbit
Reactivity
Human, Mouse
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Clonality
Polyclonal
Isotype
IgG
Specificity
ECD Antibody detects endogenous levels of ECD.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
644
Applicable Applications for anti-ECD antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human ECD.
Tissue Specificity
Highly expressed in muscle and heart. Over-expressed in pancreatic and breast cancers.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using ECD Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using ECD Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from MCF7, using ECD Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from MCF7, using ECD Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-ECD antibody
Description: Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP (PubMed:16849563, PubMed:23880345). May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes (PubMed:19919181, PubMed:9928932). Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins (PubMed:19640839). The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex (PubMed:26711270). May play a role in regulation of pre-mRNA splicing (PubMed:24722212).
Post Translational Modifications: Phosphorylated predominantly by CK2 on two serine-containing clusters; involved in cell cycle regulation activity.
Subunit Structure: Interacts with TP53, MDM2, TXNIP (PubMed:16849563, PubMed:23880345). Interacts (phosphorylated) with PIH1D1. Interacts with RUVBL1 mediating the PIH1D1-independent association with the R2TP complex (PubMed:24656813, PubMed:26711270). Interacts with RB1, RBL1 and RBL2; ECD competes with E2F1 for binding to hypophospshorylated RB1 (PubMed:19640839). Interacts with EP300 (PubMed:19919181).
Similarity: Belongs to the ECD family.
Post Translational Modifications: Phosphorylated predominantly by CK2 on two serine-containing clusters; involved in cell cycle regulation activity.
Subunit Structure: Interacts with TP53, MDM2, TXNIP (PubMed:16849563, PubMed:23880345). Interacts (phosphorylated) with PIH1D1. Interacts with RUVBL1 mediating the PIH1D1-independent association with the R2TP complex (PubMed:24656813, PubMed:26711270). Interacts with RB1, RBL1 and RBL2; ECD competes with E2F1 for binding to hypophospshorylated RB1 (PubMed:19640839). Interacts with EP300 (PubMed:19919181).
Similarity: Belongs to the ECD family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed: 73 kDa
Predicted: 73 kDa
Predicted: 73 kDa
NCBI Official Full Name
Protein ecdysoneless homolog
NCBI Official Synonym Full Names
ecdysoneless cell cycle regulator
NCBI Official Symbol
ECD
NCBI Official Synonym Symbols
GCR2; SGT1; HSGT1
NCBI Protein Information
protein ecdysoneless homolog
UniProt Protein Name
Protein SGT1
Protein Family
UniProt Gene Name
ECD
UniProt Synonym Gene Names
SGT1; hSGT1
UniProt Entry Name
SGT1_HUMAN
Uniprot Description
SGT1: Novel regulator of p53 stability and function. May also be a transcriptional activator required for the expression of glycolytic genes. Belongs to the SGT1 family.
Protein type: Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 10q22.3
Cellular Component: nucleoplasm; cytoplasm
Molecular Function: protein binding; transcription coactivator activity
Biological Process: transcription from RNA polymerase II promoter; cell proliferation; regulation of transcription, DNA-dependent; regulation of glycolysis
Research Articles on ECD
Similar Products
Product Notes
The ECD ecd (Catalog #AAA9611719) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ECD Antibody reacts with Human, Mouse Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's ECD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the ECD ecd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ECD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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