Rabbit RapGEF1 Polyclonal Antibody | anti-RapGEF1 antibody

RapGEF1 Antibody

Cat. Num. AAA9613476

• Gene Names: RAPGEF1; C3G; GRF2
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (91%), Rabbit (91%), Dog (100%), Chicken (100%), Xenopus (82%)
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: RapGEF1; Polyclonal Antibody; RapGEF1 Antibody; C3G; C3G protein; CRK SH3 binding GNRP; CRK SH3-binding GNRP; DKFZp781P1719; GRF 2; GRF2; Guanine nucleotide releasing factor 2 (specific for crk proto oncogene); Guanine nucleotide releasing factor 2; Guanine nucleotide-releasing factor 2; OTTHUMP00000064558; Protein C3G; Rap guanine nucleotide exchange factor (GEF) 1; Rap guanine nucleotide exchange factor 1; RAPGEF 1; RAPGEF1; RPGF1_HUMAN; anti-RapGEF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (91%), Rabbit (91%), Dog (100%), Chicken (100%), Xenopus (82%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: RapGEF1 Antibody detects endogenous levels of total RapGEF1.; Tissue Specificity: Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-RapGEF1 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human RapGEF1, corresponding to a region within C-terminal amino acids.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylation at Tyr-504 enhances activity as Rap guanine nucleotide exchange factor.
• Subunit Structure: Interacts with CRK via its SH3-binding sites.
• Subcellular Location: Early endosome.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HeLa  , using RapGEF1 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-RapGEF1 antibody

Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Plays a role in the establishment of basal endothelial barrier function. Plays a role in nerve growth factor (NGF)-induced sustained activation of Rap1 and neurite outgrowth.

NCBI and Uniprot Product Information

• NCBI GI #: 38373675
• NCBI GeneID: 2889
• NCBI Accession #: NP_005303.2
• NCBI GenBank Nucleotide #: NM_005312.2
• UniProt Accession #: Q13905; Q5JUE4; Q8IV73
• Molecular Weight: 120,548 Da
• NCBI Official Full Name: rap guanine nucleotide exchange factor 1 isoform a
• NCBI Official Synonym Full Names: Rap guanine nucleotide exchange factor (GEF) 1
• NCBI Official Symbol: RAPGEF1
• NCBI Official Synonym Symbols: C3G; GRF2
• NCBI Protein Information: rap guanine nucleotide exchange factor 1; CRK SH3-binding GNRP; guanine nucleotide-releasing factor 2 (specific for crk proto-oncogene)
• UniProt Protein Name: Rap guanine nucleotide exchange factor 1
• Protein Family: Rap guanine nucleotide exchange factor
• UniProt Gene Name: RAPGEF1
• UniProt Synonym Gene Names: GRF2
• UniProt Entry Name: RPGF1_HUMAN

NCBI Description

This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

RAPGEF1: an adaptor-type Src homology (SH)2-containing molecule. A guanine nucleotide releasing protein for Ras. Expressed ubiquitously in human adult and fetal tissues. Several alternatively spliced transcript variants have been described.

Protein type: Motility/polarity/chemotaxis; GEFs; GEFs, Ras

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: early endosome; cytosol; endosome

Molecular Function: protein binding; Rap guanyl-nucleotide exchange factor activity; SH3 domain binding

Biological Process: blood vessel development; cell-cell adhesion; negative regulation of protein kinase B signaling cascade; nerve growth factor receptor signaling pathway; activation of MAPKK activity; negative regulation of Ras protein signal transduction; platelet-derived growth factor receptor signaling pathway; signal transduction; Rap protein signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway