Mouse anti-Human Dynamin-1 Monoclonal Antibody | anti-Dnm1 antibody

Anti-Dynamin-1 Antibody

Cat. Num. AAA418383

• Gene Names: Dnm1; Dnm
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Crude ascites.
• Synonyms: Dynamin-1; Monoclonal Antibody; Anti-Dynamin-1 Antibody; anti-Dnm1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Purity/Purification: Crude ascites.
• Form/Format: Liquid
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 851

• Applicable Applications for anti-Dnm1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: Ni-NTA purified truncated recombinant Dynamin-1 expressed in E Coli strain BL21 (DE3)
• Storage Buffer: PBS, pH 7.4 with 0.05% sodium azide.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

Immunohistochemistry (IHC)

Related Product Information for anti-Dnm1 antibody

Dynamin-1 (Dyn1), with 864-amino acid protein (about 95kDa), belongs to the dynamin family. Dynamin-1 (neuron-specific), dynamin-2 (ubiquitously expressed), and dynamin-3 (expressed only in the testis, brain, and lung), constitute the dynamin family. Members of the dynamin family are GPTase, microtubule-associated proteins which are involved in endocytosis, synaptic transmission and neurogenesis. Dynamin-1 is phosphorylated in nerve terminals exclusively in the cytosolic compartment and in vitro by protein kinase C. Dynamin-1 is a large GTPase enzyme required in membrane constriction and fission during multiple forms of endocytosis. Dynamin-1 is also a key molecule required for the recycling of synaptic vesicles in neurons, and it has been known that dynamin-1 gene expression is induced during neuronal differentiation.

References

1.Annie Quan and Phillip J. Robinson Methods Enzymol. 2005; 404:556-69. 2.Jiyun Yoo, Moon-Jin Jeong, Byoung-Mog Kwon J. Biol. Chem., Mar 2002; 277: 11904 - 11909

NCBI and Uniprot Product Information

• NCBI GI #: 18093102
• NCBI GeneID: 140694
• NCBI Accession #: NP_542420.1
• NCBI GenBank Nucleotide #: NM_080689.4
• UniProt Accession #: P21575
• Molecular Weight: 96,399 Da
• NCBI Official Full Name: dynamin-1
• NCBI Official Synonym Full Names: dynamin 1
• NCBI Official Symbol: Dnm1
• NCBI Official Synonym Symbols: Dnm
• NCBI Protein Information: dynamin-1
• UniProt Protein Name: Dynamin-1
• Protein Family: Dynamin
• UniProt Gene Name: Dnm1
• UniProt Synonym Gene Names: Dnm

NCBI Description

mediates GTP hydrolysis; plays a role in receptor mediated endocytosis; required for agonist-induced internalization of the epidermal growth factor receptor [RGD, Feb 2006]

Uniprot Description

DYN1: a microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Concentrates within the presynaptic compartment and may participate in specialized neuronal functions such as rapid synaptic vesicle recycling. Part of a protein network that controls nucleation of actin from membranes. Contains one PH domain.

Protein type: EC 3.6.5.5; Hydrolase; Microtubule-binding; Motility/polarity/chemotaxis; Motor; Vesicle

Chromosomal Location of Human Ortholog: 3p12

Cellular Component: cytoplasm; Golgi apparatus; membrane coat; mitochondrial membrane; myelin sheath; photoreceptor inner segment; plasma membrane; protein complex; synapse; synaptic vesicle

Molecular Function: D2 dopamine receptor binding; GTPase activity; identical protein binding; microtubule binding; nitric-oxide synthase binding; protein binding; protein C-terminus binding; protein complex binding; protein dimerization activity; protein kinase binding

Biological Process: adult locomotory behavior; endocytosis; endosome organization and biogenesis; G-protein coupled receptor internalization; mitochondrial fission; protein tetramerization; receptor internalization; receptor-mediated endocytosis; sensory perception of sound; synaptic transmission, GABAergic