Rat Type I iodothyronine deiodinase ELISA Kit | Dio1 elisa kit

Rat Type I iodothyronine deiodinase ELISA Kit

Cat. Num. AAA761949

• Gene Names: Dio1; 5DI; ITDI1; TXDI1
• Reactivity: Rat
• Synonyms: Type I iodothyronine deiodinase; Rat Type I iodothyronine deiodinase ELISA Kit; Dio1/BYE1/C20orf158/DATF1/DIO1/BYE1/C20orf158/DATF-1/DATF1DIDO3/death associated transcription factor 1/death inducer-obliterator 1/Death-associated transcription factor 1/death-inducer obliterator 1/DIO1DIDO2/DIO-1hDido1/dJ885L7.8/DKFZp434P1115/FLJ11265/KIAA0333chromosome 20 open reading frame 158/MGC16140; Dio1 elisa kit

• Reactivity: Rat
• Sequence Length: 257

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Dio1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Dio1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Dio1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Dio1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Dio1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 51988898
• NCBI GeneID: 25430
• NCBI Accession #: NP_067685.5
• NCBI GenBank Nucleotide #: NM_021653.3
• Molecular Weight: 29,730 Da
• NCBI Official Full Name: type I iodothyronine deiodinase
• NCBI Official Synonym Full Names: deiodinase, iodothyronine, type I
• NCBI Official Symbol: Dio1
• NCBI Official Synonym Symbols: 5DI; ITDI1; TXDI1
• NCBI Protein Information: type I iodothyronine deiodinase
• UniProt Protein Name: Type I iodothyronine deiodinase
• Protein Family: Type I iodothyronine deiodinase
• UniProt Gene Name: Dio1
• UniProt Synonym Gene Names: Itdi1; Txdi1
• UniProt Entry Name: IOD1_RAT

NCBI Description

The protein encoded by this gene belongs to the iodothyronine deiodinase family. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). It also degrades both hormones by inner ring deiodination (IRD). This protein contains a selenocysteine (Sec) residue, which is necessary for maximal enzyme activity. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Conversion of T4 to T3 is impaired in experimental selenium deficiency, suggesting an essential role for this trace element in thyroid hormone action. [provided by RefSeq, Jul 2008]

Uniprot Description

Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine) and of T3 into T2 (3,3'-diiodothyronine).