Rabbit anti-Human, Mouse DRAK1 Polyclonal Antibody | anti-DRAK1 antibody

DRAK1, NT (Serine/Threonine protein Kinase 17A, DAP Kinase-related Apoptosis-inducing Protein Kinase 1, STK17A) (PE)

Cat. Num. AAA6472999

• Gene Names: STK17A; DRAK1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: DRAK1; Polyclonal Antibody; NT (Serine/Threonine protein Kinase 17A; DAP Kinase-related Apoptosis-inducing Protein Kinase 1; STK17A) (PE); anti-DRAK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DRAK1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-DRAK1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 in the N-terminal region of human DRAK1 UniProt Accession # Q9UEE5.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse thymus tissue lysate (35ug/lane) usingMBS625481. This demonstrates that detected the DRAK1 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in SK-BR-3 cell line lysates (35ug/lane) usingMBS625481. This demonstrates that detected the DRAK1 protein (arrow).)

Product Categories/Family for anti-DRAK1 antibody

Antibodies; Protein Kinases

References

1. Sanjo, H., et al., J. Biol. Chem. 273(44):29066-29071 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 109255245
• NCBI GeneID: 9263
• NCBI Accession #: NP_004751.2
• NCBI GenBank Nucleotide #: NM_004760.2
• UniProt Accession #: Q9UEE5; Q8IVC8; A4D1V6
• Molecular Weight: 46,558 Da
• NCBI Official Full Name: serine/threonine-protein kinase 17A
• NCBI Official Synonym Full Names: serine/threonine kinase 17a
• NCBI Official Symbol: STK17A
• NCBI Official Synonym Symbols: DRAK1
• NCBI Protein Information: serine/threonine-protein kinase 17A; DAP kinase-related apoptosis-inducing protein kinase 1; death-associated protein kinase-related 1; serine/threonine kinase 17a (apoptosis-inducing)
• UniProt Protein Name: Serine/threonine-protein kinase 17A
• UniProt Gene Name: STK17A
• UniProt Synonym Gene Names: DRAK1
• UniProt Entry Name: ST17A_HUMAN

NCBI Description

This gene is a member of the DAP kinase-related apoptosis-inducing protein kinase family and encodes an autophosphorylated nuclear protein with a protein kinase domain. The protein has apoptosis-inducing activity. [provided by RefSeq, Jul 2008]

Uniprot Description

DRAK1: a calmodulin-dependent kinase of the DAPK family. Interacts with and is regulated by calcineurin homologous protein (CHP), an EF-hand Ca(2+)-binding protein. In vitro, CHP inhibits the apoptosis-inducing protein kinase DRAK2 in the presence of elevated Ca(2+) levels. Highly expressed in placenta, lung, pancreas. Lower levels in heart, brain, liver, skeletal muscle and kidney.

Protein type: Kinase, protein; Protein kinase, CAMK; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); CAMK group; DAPK family

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: nucleus

Molecular Function: protein serine/threonine kinase activity; ATP binding

Biological Process: apoptosis; positive regulation of apoptosis; protein amino acid phosphorylation