Rabbit anti-Human DPYSL3 Polyclonal Antibody | anti-DPYSL3 antibody

DPYSL3, CT (DPYSL3, CRMP4, DRP3, ULIP, ULIP1, Dihydropyrimidinase-related protein 3, Collapsin response mediator protein 4, Unc-33-like phosphoprotein 1) (Biotin)

Cat. Num. AAA6293457

• Gene Names: DPYSL3; DRP3; ULIP; CRMP4; DRP-3; LCRMP; CRMP-4; ULIP-1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DPYSL3; Polyclonal Antibody; CT (DPYSL3; CRMP4; DRP3; ULIP; ULIP1; Dihydropyrimidinase-related protein 3; Collapsin response mediator protein 4; Unc-33-like phosphoprotein 1) (Biotin); Unc-33-like phosphoprotein 1; anti-DPYSL3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-DPYSL3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DPYSL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 460-490 amino acids from the C-terminal region of human DPYSL3.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DPYSL3 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the DPYSL3 antibody detected the DPYSL3 protein (arrow).)

Western Blot (WB)

(Western blot analysis of DPYSL3 (arrow) using rabbit polyclonal DPYSL3 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DPYSL3 gene.)

Immunohistochemistry (IHC)

(DPYSL3 Antibody (C-term) (AP14234b)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DPYSL3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-DPYSL3 antibody

Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration (By similarity).

Product Categories/Family for anti-DPYSL3 antibody

Antibodies; Enzymes

References

Okita, K., et al. Cancer Sci. 101(11):2333-2340(2010). Gao, X., et al. Oncogene 29(32):4555-4566(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 4503379
• NCBI GeneID: 1809
• NCBI Accession #: NP_001378.1
• NCBI GenBank Nucleotide #: NM_001387.2
• UniProt Accession #: Q14195; Q93012; B3SXQ8
• Molecular Weight: 61,963 Da
• NCBI Official Full Name: dihydropyrimidinase-related protein 3 isoform 2
• NCBI Official Synonym Full Names: dihydropyrimidinase-like 3
• NCBI Official Symbol: DPYSL3
• NCBI Official Synonym Symbols: DRP3; ULIP; CRMP4; DRP-3; LCRMP; CRMP-4; ULIP-1
• NCBI Protein Information: dihydropyrimidinase-related protein 3; unc-33-like phosphoprotein 1; collapsin response mediator protein 4 long
• UniProt Protein Name: Dihydropyrimidinase-related protein 3
• Protein Family: Dihydropyrimidinase-related protein
• UniProt Gene Name: DPYSL3
• UniProt Synonym Gene Names: CRMP4; DRP3; ULIP; ULIP1; DRP-3; CRMP-4; ULIP-1
• UniProt Entry Name: DPYL3_HUMAN

Uniprot Description

CRMP-4: Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration. Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: filamentous actin; extracellular space; growth cone; lamellipodium; cytosol

Molecular Function: hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides; phosphoprotein binding; SH3 domain binding

Biological Process: actin crosslink formation; positive regulation of filopodium formation; axon guidance; actin filament bundle formation; response to axon injury; negative regulation of cell migration; pyrimidine base catabolic process; protein homooligomerization