Rabbit AVP Polyclonal Antibody | anti-AVP antibody

AVP Polyclonal Antibody

Cat. Num. AAA3007519

• Gene Names: AVP; VP; ADH; ARVP; AVRP; AVP-NPII
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: AVP; Polyclonal Antibody; AVP Polyclonal Antibody; Vasopressin-neurophysin 2-copeptin; AVP-NPII; Arginine-vasopressin; Neurophysin-II; anti-AVP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: AVP polyclonal antibody detects endogenous levels of AVP protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 164

• Applicable Applications for anti-AVP antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding Human AVP.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of AVP polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:COS-7 whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:CT26 whole cell lysate (40ug))

Related Product Information for anti-AVP antibody

Vasopressin is a neuroendocrine peptide that is released to the circulation by magnocellular neurons whose cell bodies are mainly found in the paraventricular and the supraoptic nuclei of the hypothalamus. It was first isolated from pituitary gland extracts and synthesized in 1951. Vasopressin acts by activating G protein-coupled, V1a, V1b (also known as V3) and V2 receptors and plays a fundamental role in the maintenance of water homeostasis. One of its main functions is body water retention, hence its alternative name antidiuretic hormone or ADH. Vasopressin also leads to increased arterial blood pressure by raising peripheral vascular resistance. Vasopressin is also involved in other physiological processes such as acute heart failure, pain, and metabolic syndrome.

NCBI and Uniprot Product Information

• NCBI GI #: 13259533
• NCBI GeneID: 551
• NCBI Accession #: NP_000481.2
• NCBI GenBank Nucleotide #: NP_000481.2
• UniProt Accession #: P01185
• Molecular Weight: ~ 17kDa
• NCBI Official Full Name: vasopressin-neurophysin 2-copeptin preproprotein
• NCBI Official Synonym Full Names: arginine vasopressin
• NCBI Official Symbol: AVP
• NCBI Official Synonym Symbols: VP; ADH; ARVP; AVRP; AVP-NPII
• NCBI Protein Information: vasopressin-neurophysin 2-copeptin
• UniProt Protein Name: Vasopressin-neurophysin 2-copeptin
• Protein Family: Neurophysin
• UniProt Gene Name: AVP
• UniProt Synonym Gene Names: ARVP; VP
• UniProt Entry Name: NEU2_HUMAN

NCBI Description

This gene encodes a member of the vasopressin/oxytocin family and preproprotein that is proteolytically processed to generate multiple protein products. These products include the neuropeptide hormone arginine vasopressin, and two other peptides, neurophysin 2 and copeptin. Arginine vasopressin is a posterior pituitary hormone that is synthesized in the supraoptic nucleus and paraventricular nucleus of the hypothalamus. Along with its carrier protein, neurophysin 2, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis where it is either stored or secreted into the bloodstream. The precursor is thought to be activated while it is being transported along the axon to the posterior pituitary. Arginine vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney, and also causes vasoconstriction of the peripheral vessels. This hormone can contract smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in this gene cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI). This gene is present in a gene cluster with the related gene oxytocin on chromosome 20. [provided by RefSeq, Nov 2015]

Uniprot Description

AVP: Neurophysin 2 specifically binds vasopressin. Defects in AVP are the cause of diabetes insipidus, neurohypophyseal (NDI). A disease characterized by persistent thirst, polydipsia and polyuria. Affected individuals are apparently normal at birth, but characteristically develop symptoms of vasopression deficiency during childhood. Belongs to the vasopressin/oxytocin family.

Protein type: Hormone; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: extracellular space; dendrite; extracellular region; cytosol; secretory granule

Molecular Function: neuropeptide hormone activity; signal transducer activity; V1A vasopressin receptor binding; caspase inhibitor activity; neurohypophyseal hormone activity; V1B vasopressin receptor binding; receptor binding; protein kinase activity

Biological Process: response to nicotine; circadian rhythm; water transport; positive regulation of glutamate secretion; positive regulation of cellular pH reduction; locomotory behavior; positive regulation of systemic arterial blood pressure; negative regulation of caspase activity; signal transduction; protein amino acid phosphorylation; elevation of cytosolic calcium ion concentration; cell-cell signaling; hyperosmotic salinity response; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; transmembrane transport; positive regulation of prostaglandin biosynthetic process; penile erection; grooming behavior; vasoconstriction; sodium-independent organic anion transport; generation of precursor metabolites and energy; maternal behavior; response to testosterone stimulus; social behavior; positive regulation of cell growth; positive regulation of peptidyl-serine phosphorylation; negative regulation of female receptivity; response to ethanol; negative regulation of transmission of nerve impulse; positive regulation of vasoconstriction; renal water homeostasis; negative regulation of apoptosis

Disease: Diabetes Insipidus, Neurohypophyseal