Human Dopa Decarboxylase (DDC) ELISA Kit | DDC elisa kit

Human Dopa Decarboxylase (DDC) ELISA Kit

Cat. Num. AAA450861

• Gene Names: DDC; AADC
• Reactivity: Human
• Synonyms: Dopa Decarboxylase (DDC); Human Dopa Decarboxylase (DDC) ELISA Kit; AADC; Aromatic L-Amino Acid Decarboxylase; DOPA decarboxylase; DDC elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of DDC. No significant cross-reactivity or interference between DDC and analogues was observed.
• Sequence Length: 480

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.068ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DDC were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DDC were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DDC elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DDC in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DDC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DDC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DDC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DDC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4503281
• NCBI GeneID: 1644
• NCBI Accession #: NP_000781.1
• NCBI GenBank Nucleotide #: NM_000790.3
• UniProt Accession #: P20711; Q16723; Q5W5T9; Q75MJ6; C9IYA0; E7ER62; E7EU95
• Molecular Weight: 44,239 Da
• NCBI Official Full Name: aromatic-L-amino-acid decarboxylase isoform 1
• NCBI Official Synonym Full Names: dopa decarboxylase
• NCBI Official Symbol: DDC
• NCBI Official Synonym Symbols: AADC
• NCBI Protein Information: aromatic-L-amino-acid decarboxylase
• UniProt Protein Name: Aromatic-L-amino-acid decarboxylase
• Protein Family: Aromatic-L-amino-acid decarboxylase
• UniProt Gene Name: DDC
• UniProt Synonym Gene Names: AADC; AADC; DDC

NCBI Description

The encoded protein catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Defects in this gene are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2011]

Uniprot Description

Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.