Rabbit anti-Human, Mouse DOC-1 / CDK2AP1 Polyclonal Antibody | anti-DOC-1 antibody

Rabbit Polyclonal (IgG) to Human DOC-1 / CDK2AP1

Cat. Num. AAA248771

• Gene Names: CDK2AP1; DOC1; ST19; DORC1; doc-1; p12DOC-1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: DOC-1 / CDK2AP1; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human DOC-1 / CDK2AP1; Anti-DOC-1 / CDK2AP1 Antibody (aa51-100) IHC-plus; CDK2AP1; Doc-1; DORC1; Deleted in oral cancer 1; Deleted in oral cancer-1; p12DOC-1; ST19; CDK2-associated protein 1; CDKAP1; DOC1; Human DOC-1; anti-DOC-1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CDKAP1 Antibody detects endogenous levels of total CDKAP1 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Sequence Length: 115

• Applicable Applications for anti-DOC-1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:1000), IHC-P (10 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human CDKAP1 (aa51-100).
• Immunogen: DOC-1 / CDK2AP1 antibody was raised against synthetic peptide from human CDKAP1 (aa51-100).
• Antigen Modification: aa51-100
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Human, Small Intestine: Formalin-Fixed Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot of extracts from HeLa cells, using CDKPA1 Antibody. The lane on the right is treated with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 4758188
• NCBI GeneID: 8099
• NCBI Accession #: NP_004633.1
• NCBI GenBank Nucleotide #: NM_004642.3
• UniProt Accession #: O14519; F5GYA4
• Molecular Weight: 9,601 Da
• NCBI Official Full Name: cyclin-dependent kinase 2-associated protein 1 isoform 1
• NCBI Official Synonym Full Names: cyclin-dependent kinase 2 associated protein 1
• NCBI Official Symbol: CDK2AP1
• NCBI Official Synonym Symbols: DOC1; ST19; DORC1; doc-1; p12DOC-1
• NCBI Protein Information: cyclin-dependent kinase 2-associated protein 1; Deleted in oral cancer-1; deleted in oral cancer 1; CDK2-associated protein 1; putative oral cancer suppressor
• UniProt Protein Name: Cyclin-dependent kinase 2-associated protein 1
• UniProt Gene Name: CDK2AP1
• UniProt Synonym Gene Names: CDKAP1; DOC1; CDK2-associated protein 1; DOC-1
• UniProt Entry Name: CDKA1_HUMAN

NCBI Description

The protein encoded by this gene is a cyclin-dependent kinase 2 (CDK2) -associated protein which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NURD) complex that epigenetically regulates embryonic stem cell differentiation. This gene thus plays a role in both cell-cycle and epigenetic regulation. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]

Uniprot Description

CDK2AP1: specific inhibitor of the cell-cycle kinase CDK2. Belongs to the CDK2AP family.

Protein type: Tumor suppressor; Cell cycle regulation; DNA replication

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: perinuclear region of cytoplasm; nucleus

Biological Process: positive regulation of protein amino acid phosphorylation; cell cycle; DNA-dependent DNA replication