Testing Data
(Antigen: Rotavirus Strain SA-11, MBS655304, coated at 1ug/well.Antibody: Dilution Series of MBS631538, incubated at 2 hours room temperature followed by IgG (HRP) goat anti-mouse and OPD substrate.)
Mouse Rotavirus VP6 Monoclonal Antibody
Rotavirus VP6
Applications
ELISA
Purity
Purified by Protein A affinity chromatography.
Synonyms
Rotavirus VP6; Monoclonal Antibody; Anti -Rotavirus VP6; anti-Rotavirus VP6 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
10K13
Specificity
Recognizes Rotavirus group A specific antigen VP6. Reacts with strains EDIM, SA-11, Wa, and bovine NCDV.
Purity/Purification
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Concentration
5.11 mg/ml (varies by lot)
Applicable Applications for anti-Rotavirus VP6 antibody
ELISA (EL/EIA)
Application Notes
Recommended Dilution:
Indirect ELISA: Use MBS655304 as the antigen, coated at 1ug/well. Incubate for 2 hours at RT with MBS631538 as the primary antibody.
Use a suitable secondary IgG (HRP) goat anti-mouse and OPD as the substrate.
Optimal dilutions to be determined by the researcher.
Indirect ELISA: Use MBS655304 as the antigen, coated at 1ug/well. Incubate for 2 hours at RT with MBS631538 as the primary antibody.
Use a suitable secondary IgG (HRP) goat anti-mouse and OPD as the substrate.
Optimal dilutions to be determined by the researcher.
Immunogen
Murine Rotavirus EDIM
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Testing Data
(Antigen: Rotavirus Strain SA-11, MBS655304, coated at 1ug/well.Antibody: Dilution Series of MBS631538, incubated at 2 hours room temperature followed by IgG (HRP) goat anti-mouse and OPD substrate.)
Testing Data
(Antigen: Rotavirus Strain SA-11, MBS655304, coated at 1ug/well.Antibody: Dilution Series of MBS631538, incubated at 2 hours room temperature followed by IgG (HRP) goat anti-mouse and OPD substrate.)
Related Product Information for anti-Rotavirus VP6 antibody
Rotaviruses, members of the family Reoviridae, are a major cause of diarrhea in young mammals. Rotavirus infections also result in economic losses in agriculture due to diarrhea in calf, pig, sheep, and poultry rearing. Diarrhea (or scours) due to the rotavirus Nebraska Calf Diarrhea Virus can affect calves up to 30 days of age or older. Diarrhea begins 2 to 3 days after exposure. identification is by history, lesions (ulcers on the tongue, lips, and mouth) and laboratory tests. Mortality rates may be as high as 50 percent, depending on the secondary bacteria present.
Human rotaviruses, the major etiological agents of severe infantile diarrhea worldwide, display very diverse and complex serotypic specificities. Rotaviruses are 70 nm, non enveloped viruses comprised of a triple layered protein capsid; Outer capsid proteins are VP4 and VP7, Inner capsid -VP6 and Core -VP2. The immunity acquired from exposure to rotavirus appears to be type specific following initial infection. Therefore, multiple serotypes of rotavirus mean multiple opportunities for infection. The combination of animal reservoirs for the virus and rotavirus gene reassortment provides the potential for dramatic genetic shifts (similar to influenza virus) which could give rise to altered host ranges and viral virulence.
Human rotaviruses, the major etiological agents of severe infantile diarrhea worldwide, display very diverse and complex serotypic specificities. Rotaviruses are 70 nm, non enveloped viruses comprised of a triple layered protein capsid; Outer capsid proteins are VP4 and VP7, Inner capsid -VP6 and Core -VP2. The immunity acquired from exposure to rotavirus appears to be type specific following initial infection. Therefore, multiple serotypes of rotavirus mean multiple opportunities for infection. The combination of animal reservoirs for the virus and rotavirus gene reassortment provides the potential for dramatic genetic shifts (similar to influenza virus) which could give rise to altered host ranges and viral virulence.
Product Categories/Family for anti-Rotavirus VP6 antibody
Similar Products
Product Notes
The Rotavirus VP6 (Catalog #AAA631538) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Rotavirus VP6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA). Recommended Dilution: Indirect ELISA: Use MBS655304 as the antigen, coated at 1ug/well. Incubate for 2 hours at RT with MBS631538 as the primary antibody. Use a suitable secondary IgG (HRP) goat anti-mouse and OPD as the substrate. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Rotavirus VP6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rotavirus VP6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
