Immunofluorescence (IF)
(Fluorescent image of U251 cell stained with TAF9 Antibody (N-term). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TAF9 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).TAF9 immunoreactivity is localized to Nucleus significantly.)
Rabbit anti-Human TAF9 Polyclonal Antibody | anti-TAF9 antibody
TAF9 Antibody (N-term)
WB~~1:1000
Immunofluorescence (IF)
(Fluorescent image of U251 cell stained with TAF9 Antibody (N-term). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TAF9 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).TAF9 immunoreactivity is localized to Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent image of U251 cell stained with TAF9 Antibody (N-term). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TAF9 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).TAF9 immunoreactivity is localized to Nucleus significantly.)
Western Blot (WB)
(TAF9 Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the TAF9 antibody detected the TAF9 protein (arrow).)
Western Blot (WB)
(TAF9 Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the TAF9 antibody detected the TAF9 protein (arrow).)
the activities of more than 70 polypeptides. The protein that
coordinates these activities is transcription factor IID (TFIID),
which binds to the core promoter to position the polymerase
properly, serves as the scaffold for assembly of the remainder of
the transcription complex, and acts as a channel for regulatory
signals. TFIID is composed of the TATA-binding protein (TBP) and a
group of evolutionarily conserved proteins known as TBP-associated
factors or TAFs. TAFs may participate in basal transcription, serve
as coactivators, function in promoter recognition or modify general
transcription factors (GTFs) to facilitate complex assembly and
transcription initiation. This gene encodes one of the smaller
subunits of TFIID that binds to the basal transcription factor
GTF2B as well as to several transcriptional activators such as p53
and VP16. A similar but distinct gene (TAF9L) has been found on the
X chromosome and a pseudogene has been identified on chromosome 19.
Alternative splicing results in multiple transcript variants
encoding different isoforms.
Sengupta, T., et al. Proc. Natl. Acad. Sci. U.S.A. 106(11):4213-4218(2009)
Liu, X., et al. Mol. Cell. Biol. 28(1):108-121(2008)
McKeegan, K.S., et al. Mol. Cell. Biol. 27(19):6782-6793(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
NCBI and Uniprot Product Information
NCBI Description
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several transcriptional activators such as p53 and VP16. In human, TAF9 and AK6 (GeneID: 102157402) are two distinct genes that share 5' exons. A similar but distinct gene (TAF9L) has been found on the X chromosome and a pseudogene has been identified on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
Uniprot Description
TAFII31: a component of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TIIFD is essential for mediating regulation of RNA polymerase transcription. TAFII31 binds to p53, the basal transcription factor GTF2B, and the acidic transactivator viral protein 16 (VP16). It is a coactivator for P53.
Protein type: EC 2.7.4.3; Transcription initiation complex; Acetyltransferase; DNA-binding; Kinase, other
Chromosomal Location of Human Ortholog: 5q13.2
Cellular Component: nucleoplasm; PCAF complex; transcription factor TFIID complex
Molecular Function: protein binding; histone acetyltransferase activity; transcription activator binding; DNA binding; p53 binding; protein heterodimerization activity; transcription coactivator activity
Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; protein stabilization; viral reproduction; RNA elongation from RNA polymerase II promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of cell growth; response to DNA damage stimulus; negative regulation of apoptosis
Research Articles on TAF9
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Product Notes
The TAF9 taf9 (Catalog #AAA9207638) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-30. The TAF9 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TAF9 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Western Blot (WB). IF~~1:10~50 WB~~1:1000. Researchers should empirically determine the suitability of the TAF9 taf9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TAF9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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