Western Blot (WB)
(Western blot analysis was performed by loading 25ug of THP-1 PMA treated cells probed with Rabbit anti SMPD1/ASM at 1/1000 dilution. Arrow points to SMPD1/ASM (molecular weight 60 kDa).)
Rabbit anti-Human SMPD1 Polyclonal Antibody | anti-SMPD1 antibody
RABBIT ANTI SMPD1
Reactivity
Human
Applications
Western Blot
Synonyms
SMPD1; Polyclonal Antibody; RABBIT ANTI SMPD1; SMPD1 antibody; anti-SMPD1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
SMPD1
Form/Format
Purified IgG, liquid. Phosphate buffered saline
Antiserum to SMPD1 was raised by repeated immunization of rabbits with highly purified antigen
Antiserum to SMPD1 was raised by repeated immunization of rabbits with highly purified antigen
Applicable Applications for anti-SMPD1 antibody
Western Blot (WB)
Application Notes
WB: 1/500-1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Target Species
Human
Species Cross Reactivity
Reacts with: Rat, Mouse
N.B. Antibody reactivity and working conditions may vary between species. Cross reactivity is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
N.B. Antibody reactivity and working conditions may vary between species. Cross reactivity is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
Immunogen
Recombinant protein of human SMPD1 / ASM
Preservative Stabilizers
0.02% Sodium Azide
50% Glycerol
50% Glycerol
Preparation and Storage
`Store at -20 degree C
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Western Blot (WB)
(Western blot analysis was performed by loading 25ug of THP-1 PMA treated cells probed with Rabbit anti SMPD1/ASM at 1/1000 dilution. Arrow points to SMPD1/ASM (molecular weight 60 kDa).)
Western Blot (WB)
(Western blot analysis was performed by loading 25ug of THP-1 PMA treated cells probed with Rabbit anti SMPD1/ASM at 1/1000 dilution. Arrow points to SMPD1/ASM (molecular weight 60 kDa).)
Western Blot (WB)
(Western blot analysis was performed by loading 25ug of various whole cell lysates probed with Rabbit anti SMPD1/ASM at 1/1000 dilution. Arrow points to SMPD1/ASM (molecular weight 60 kDa).)
Western Blot (WB)
(Western blot analysis was performed by loading 25ug of various whole cell lysates probed with Rabbit anti SMPD1/ASM at 1/1000 dilution. Arrow points to SMPD1/ASM (molecular weight 60 kDa).)
Related Product Information for anti-SMPD1 antibody
Rabbit anti SMPD1 antibody recognizes sphingomyelin phosphodiesterase, also known as ASM, Sphingomyelin Phosphodiesterase, ASMASE and NPD.
It is a lipid hydrolase that converts sphingomyelin to the bioactive messenger ceramide, and is activated by a number of molecules including TNF-alpha and LPS (Rozanova et al. 2010). Mutations in the SMPD1 gene resulting in deficient ASM production cause Niemann-Pick disease, one type of which causes lethality within three years of life (Schuchman 2007). SMPD1 mutations have also been implicated in sporadic Parkinson's disease (Mao et al. 2017). A role of ASM on mood regulation has been suggested, with high ASM expression implicated in major depressive episodes (Gulbins et al. 2015).
It is a lipid hydrolase that converts sphingomyelin to the bioactive messenger ceramide, and is activated by a number of molecules including TNF-alpha and LPS (Rozanova et al. 2010). Mutations in the SMPD1 gene resulting in deficient ASM production cause Niemann-Pick disease, one type of which causes lethality within three years of life (Schuchman 2007). SMPD1 mutations have also been implicated in sporadic Parkinson's disease (Mao et al. 2017). A role of ASM on mood regulation has been suggested, with high ASM expression implicated in major depressive episodes (Gulbins et al. 2015).
Similar Products
Product Notes
The SMPD1 (Catalog #AAA225930) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RABBIT ANTI SMPD1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SMPD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1/500-1/2000 Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the SMPD1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMPD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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