Rabbit anti-Human, Mouse ANXA7 Polyclonal Antibody | anti-ANXA7 antibody

ANXA7 Polyclonal Antibody

Cat. Num. AAA3007113

• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ANXA7; Polyclonal Antibody; ANXA7 Polyclonal Antibody; Annexin A7; Annexin VII; Annexin-7; Synexin; ANX7; SNX; anti-ANXA7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ANXA7 polyclonal antibody detects endogenous levels of ANXA7 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 488

• Applicable Applications for anti-ANXA7 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: A synthetic peptide corresponding to residues in Human ANXA7.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ANXA7 polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysateLane2:sp2/0 whole cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ANXA7 pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing cytoplasm,nuclear and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-ANXA7 antibody

The annexin family of calcium-binding proteins is composed of at least ten mammalian genes. It is characterized by a conserved core domain, which binds to phospholipids in a Ca++-dependent manner, and a unique amino terminal region, which may confer binding specificity. The annexin family has been implicated as regulators of such diverse processes as ion-flux, endocytosis and exocytosis, and cellular adhesion. When overexpressed in A431 cells, Annexin VI causes a partial reversal of the transformed phenotype. It has been hypothesized that growth-dependent post-translational modifications of Annexins are required for proper sub-cellular localization Annexin VII, also referred to as synexin, is located at the plasma membrane in normal muscle tissue. However, in muscle samples from patients suffering from Duchennes muscular dystrophy, Annexin VII, along with Annexins IV and VI, is released into the cytoplasm and later, as the disease progresses, into the extracellular space. Two forms of Annexin XI, designated A and B, have been identified. Transfection of COS-7 cells with Annexin XI-A, but not Annexin XI-B, causes formation of Annexin XI-associated vesicles.

NCBI and Uniprot Product Information

• NCBI GI #: 215274186
• UniProt Accession #: P20073
• Molecular Weight: ~ 53kDa
• NCBI Official Full Name: Annexin A7
• UniProt Protein Name: Annexin A7
• Protein Family: Annexin
• UniProt Gene Name: ANXA7
• UniProt Synonym Gene Names: ANX7; SNX
• UniProt Entry Name: ANXA7_HUMAN

Uniprot Description

ANXA7: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. Interacts with PDCD6. Isoform 1 is expressed in brain, heart and skeletal muscle. Isoform 2 is more abundant in liver, lung, kidney, spleen, fibroblasts and placenta. Belongs to the annexin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Calcium-binding; Motility/polarity/chemotaxis; Lipid-binding

Chromosomal Location of Human Ortholog: 10q22.2

Cellular Component: endoplasmic reticulum membrane; membrane; plasma membrane; nuclear envelope; cytosol; nucleus

Molecular Function: integrin binding; protein binding; calcium-dependent phospholipid binding; calcium ion binding; calcium-dependent protein binding

Biological Process: cellular calcium ion homeostasis; regulation of cell shape; cell proliferation; cellular water homeostasis; epithelial cell differentiation; hemostasis; autophagy; social behavior; response to calcium ion; response to organic cyclic substance; response to salt stress