Rabbit DMPO Polyclonal Antibody
DMPO (5,5-dimethyl-1-pyrroline-N-oxide)
Applications
ELISA, Western Blot, Immunocytochemistry
Purity
SerumSerum
Synonyms
DMPO; Polyclonal Antibody; DMPO (5; 5-dimethyl-1-pyrroline-N-oxide); Anti -DMPO (5; anti-DMPO antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
Recognizes DMPO.
Purity/Purification
Serum
Serum
Serum
Form/Format
Supplied as a liquid.
Applicable Applications for anti-DMPO antibody
ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: Western Blot: 1:5,000
ELISA: 1:5,000 to 1:10,000
Immunohistochemistry: 1:1,000 (subject to optimization)
Dilution: Western Blot: 1:5,000
ELISA: 1:5,000 to 1:10,000
Immunohistochemistry: 1:1,000 (subject to optimization)
Immunogen
5,5-dimethyl-1-pyrroline-N-oxide (DMPO)-octanoic acid adduct (ovalbumin).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-DMPO antibody
Free radicals are highly reactive and generally short-lived species. Hence, most studies of free radicals have been restricted to analysis of the end products formed by their interaction with cellular biomolecules. One technology that has permitted direct study of free radicals is electron spin resonance (ESR). For very short lived radicals, spin traps have been empolyed to generate longer lived radicals that are more amenable to ESR analysis. The most widely used of these spin traps is 5,5-dimethyl-1-pyrroline-N-oxide (DMPO). The reaction of DMPO with a protein radical gives rise to a nitroxide radical which can be disproportionate to hydrozylamine and nitrone adducts. Of these species, only the nitrone adduct can be considered stable. It has been shown that our DMPO antibody will specifically react with radicals in protein.
DMPO reagent can be employed for the immunochemical identifcation and characterization of nitrone adducts that result from the reaction of protein radicals with DMPO by immuno-spin trapping.
DMPO reagent can be employed for the immunochemical identifcation and characterization of nitrone adducts that result from the reaction of protein radicals with DMPO by immuno-spin trapping.
Product Categories/Family for anti-DMPO antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The DMPO (Catalog #AAA622873) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DMPO can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC). Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Dilution: Western Blot: 1:5,000 ELISA: 1:5,000 to 1:10,000 Immunohistochemistry: 1:1,000 (subject to optimization). Researchers should empirically determine the suitability of the DMPO for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DMPO, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
