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Western Blot (WB) ((1ug/ml) staining of Peripheral Blood Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat TRIM29/ATDC Polyclonal Antibody | anti-TRIM29 antibody

Goat anti-TRIM29/ATDC Antibody

Gene Names
TRIM29; ATDC
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
TRIM29/ATDC; Polyclonal Antibody; Goat anti-TRIM29/ATDC Antibody; TRIM29; tripartite motif-containing 29; ATDC; FLJ36085; ataxia-telangiectasia group D-associated protein; tripartite motif protein TRIM29; ataxia-telangiectasia group D-associated protein antibody; ATDC antibody; FLJ36085 antibody; tripartite motif protein TRIM29 antibody; tripartite motif-containing 29 antibody; TRIM29 antibody; TRIM29 / ATDC; anti-TRIM29 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
EKDRIKSFTTNE
Sequence Length
588
Applicable Applications for anti-TRIM29 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Immunohistochemistry: In paraffin embedded Human Skin shows staining of subnuclear structures and cytoplasm in keratinocytes. Recommended concentration, 1-2ug/ml.
Immunogen
Peptide with sequence C-EKDRIKSFTTNE, from the internal region of the protein sequence according to NP_036233.2.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of Peripheral Blood Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((1ug/ml) staining of Peripheral Blood Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Immunohistochemistry (IHC)

((3.8ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

Immunohistochemistry (IHC) ((3.8ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63,844 Da
NCBI Official Full Name
tripartite motif-containing protein 29
NCBI Official Synonym Full Names
tripartite motif containing 29
NCBI Official Symbol
TRIM29
NCBI Official Synonym Symbols
ATDC
NCBI Protein Information
tripartite motif-containing protein 29
UniProt Protein Name
Tripartite motif-containing protein 29
UniProt Gene Name
TRIM29
UniProt Synonym Gene Names
ATDC
UniProt Entry Name
TRI29_HUMAN

NCBI Description

The protein encoded by this gene belongs to the TRIM protein family. It has multiple zinc finger motifs and a leucine zipper motif. It has been proposed to form homo- or heterodimers which are involved in nucleic acid binding. Thus, it may act as a transcriptional regulatory factor involved in carcinogenesis and/or differentiation. It may also function in the suppression of radiosensitivity since it is associated with ataxia telangiectasia phenotype. [provided by RefSeq, Jul 2008]

Uniprot Description

TRIM29: It is able to complement the radiosensitivity defect of an ataxia telangiectasia (AT) fibroblast cell line. Interacts with VIM and HINT1. Expressed in placenta, prostate and thymus. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: cytoplasm; intermediate filament cytoskeleton; nucleoplasm

Molecular Function: p53 binding; protein binding; transcription factor activity; zinc ion binding

Biological Process: negative regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter

Research Articles on TRIM29

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Product Notes

The TRIM29 trim29 (Catalog #AAA422286) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-TRIM29/ATDC Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TRIM29/ATDC can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: In paraffin embedded Human Skin shows staining of subnuclear structures and cytoplasm in keratinocytes. Recommended concentration, 1-2ug/ml. Researchers should empirically determine the suitability of the TRIM29 trim29 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EKDRIKSFTT NE. It is sometimes possible for the material contained within the vial of "TRIM29/ATDC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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