Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-DMPO Monoclonal Antibody, Clone N1664A. Tissue: macrophage cell line (Raw 264.7). Species: Mouse. Primary Antibody: Mouse Anti-DMPO Monoclonal Antibody at 1:100. Secondary Antibody: Alexa Fluor 488 Goat Anti-Mouse (green) at 1:1000. Counterstain: DAPI (blue) nuclear stain.)
Mouse DMPO Monoclonal Antibody | anti-dmpO antibody
DMPO Antibody
Reactivity
Species Independent
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Synonyms
DMPO; Monoclonal Antibody; DMPO Antibody; 5;5-dimethyl-2-(8-octanoic acid)-1-pyrroline N oxide; DMPO nitrone adduct; anti-dmpO antibody
Host
Mouse
Reactivity
Species Independent
Clonality
Monoclonal
Isotype
IgG1
Clone Number
N1664A
Form/Format
Protein G Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
119
Applicable Applications for anti-dmpO antibody
Immuno-spin trapping, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC),Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
1-10ug/mL (ELISA),10ug/mL (ICC), 25ug (IP)
Certificate of Analysis
A 1/1000 dilution was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100ng/lane by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Storage Buffer
TBS pH7.4, 50% glycerol, 0.05% sodium azide
Preparation and Storage
-20 degree C
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-DMPO Monoclonal Antibody, Clone N1664A. Tissue: macrophage cell line (Raw 264.7). Species: Mouse. Primary Antibody: Mouse Anti-DMPO Monoclonal Antibody at 1:100. Secondary Antibody: Alexa Fluor 488 Goat Anti-Mouse (green) at 1:1000. Counterstain: DAPI (blue) nuclear stain.)
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-DMPO Monoclonal Antibody, Clone N1664A. Tissue: macrophage cell line (Raw 264.7). Species: Mouse. Primary Antibody: Mouse Anti-DMPO Monoclonal Antibody at 1:100. Secondary Antibody: Alexa Fluor 488 Goat Anti-Mouse (green) at 1:1000. Counterstain: DAPI (blue) nuclear stain.)
Western Blot (WB)
(Western Blot analysis of Human HL 60 clone 15 eosinophils lysates showing detection of DMPO protein using Mouse Anti-DMPO Monoclonal Antibody, Clone N1664A. Primary Antibody: Mouse Anti-DMPO Monoclonal Antibody at 1:200.)
Western Blot (WB)
(Western Blot analysis of Human HL 60 clone 15 eosinophils lysates showing detection of DMPO protein using Mouse Anti-DMPO Monoclonal Antibody, Clone N1664A. Primary Antibody: Mouse Anti-DMPO Monoclonal Antibody at 1:200.)
Related Product Information for anti-dmpO antibody
Background Info: Detects ~90kDa. Recognzies DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA.
Scientific Background: The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states (1). The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems (2-6). Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals (1). DMPO adducts can be generated with protein and DNA radicals (7).
Scientific Background: The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states (1). The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems (2-6). Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals (1). DMPO adducts can be generated with protein and DNA radicals (7).
Product Categories/Family for anti-dmpO antibody
References
1. Reszka K.J., et al. (2006) Nitric Oxide 15: 133-141. 2. Ramirez D.C., Gomez-Mejiba S.E., and Mason R.P. (2007) Nat Protoc. 2(3): 512-522. 3. Khan N., et al. (2003) Free Radic. Biol. Med 34:1473- 1481. 4. Haseloff R.F., et al. (1997) FEBS Lett 418:73-75. 5. Schaefer C.F., Janzen E.G., West M.S., Poyer J.L., and Kosanke S.D. (1996) Free Radic. Biol. Med 21:427-436. 6. Anzai K., et al. (2003) Arch. Biochem. Biophys 415:251- 256. 7. Free Radic Biol Med. 2009 April 1; 46(7): 853-865. doi:10.1016/j.freeradbiomed.2008.12.020. 8. Chatterjee S., Ehrenshaft, M., Bhattacharjee,S., Derterding, L.J., Bonini, M.G., Corbett, J., Kadiiska, M.B., Tomer K.B. and Mason, R.P. 2009 Free Radic. Med. and Biol. 46:454-461.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
13,207 Da
NCBI Official Full Name
DmpO
UniProt Protein Name
DmpO
Protein Family
UniProt Gene Name
dmpO
UniProt Entry Name
Q68J58_PSEPU
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Product Notes
The DMPO dmpo (Catalog #AAA800097) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DMPO Antibody reacts with Species Independent and may cross-react with other species as described in the data sheet. AAA Biotech's DMPO can be used in a range of immunoassay formats including, but not limited to, Immuno-spin trapping, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC),Immunofluorescence (IF), Immunoprecipitation (IP). 1-10ug/mL (ELISA),10ug/mL (ICC), 25ug (IP). Researchers should empirically determine the suitability of the DMPO dmpo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DMPO, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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